基于多重荧光PCR检测的肉及其制品中鸭DNA成分的鉴别方法  

A method for dark DNA components detection in meat and productions by multiplex-real-time fluorescence PCR

作　　者：林霖[1] 陈国培[1] 何永盛[1] 杨国武[1] 赖心田[1] 

机构地区：[1]深圳市计量质量检测研究院,广东深圳518131

出　　处：《食品与机械》2017年第5期95-98,共4页Food and Machinery

基　　金：广东省质量技术监督局科技项目(编号:2013CZ05)

摘　　要：针对现有肉制品中鸭DNA成分检测方法不能检测番鸭DNA成分的问题,通过下载番鸭、家鸭及其相近物种的12SrDNA序列,使用CLUSTAL X2进行序列比对,筛选特异性序列,设计了一对通用引物及两条番鸭、家鸭特异性探针,构建了可同时检测番鸭、家鸭DNA成分的实时荧光PCR方法。结果表明:最终构建的检测方法可在掺伪量为0.1%的情况下,检测出样品中的家鸭或番鸭DNA成分。该方法相比普通PCR或单重荧光PCR检测方法节省了劳动力,提高了检测效率,补充了现有检测方法的不足,为更有效地识别掺伪肉类提供技术支持。The Cairina moschata DNA component can not be detected by the current duck DNA component detection methods. By downloading Cairina moschata , and Arias platyrhynchos domestica and similar species 12S rDNA sequences and compareing them by u- sing Clustal X2 software to find a specific sequence, a pair of univer- sal primers and two individual specific probes were designed based on the sequence, which construct specific detect Caivina moschata and Anas platyrhynchos domestica DNA components real-time PCR sys- tem. This detection system can detect as low as 0.1 % adulteration. Results： Using this detection system can detect Cairina tnoschata and Anas platyrhynchos dumestica DNA components from the adul- teration samples. This method, compares with normal PCR method or single real time PCR method, requires lesser labor force, and has higher detection efficiency. This method also complements the deft ciency of standard detection methods, and provides technical support for more effective identification of adulterated meat.

关 键 词：番鸭 家鸭 肉制品 实时荧光PCR DNA 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]