海河流域水体诺如病毒与新型指示微生物的相关性研究  

Correlation between norovirus and novel indicator microorganism in Haihe Rive

作　　者：李超[1] 许蕊[1,2] 杨继平[1,2] 杨栋[1] 邱志刚[1] 金敏[1] 李君文[1] 徐群英[2] 谌志强[1] 袭著革[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所天津市环境与食品安全风险监控技术重点实验室,天津300050 [2]南昌大学公共卫生学院

出　　处：《环境与健康杂志》2017年第4期309-312,共4页Journal of Environment and Health

基　　金：国家自然科学基金面上项目(81270041);国家科技支撑计划项目(2012BAJ25B03)

摘　　要：目的探讨海河流域诺如病毒与新型指示微生物——通用拟杆菌的相关性。方法于2013年12月—2014年11月对天津海河流域水中的粪大肠菌群、F(+)噬菌体、通用拟杆菌3项指示微生物和沙门菌、诺如病毒2项致病微生物进行监测。结果粪大肠菌群浓度为0~6.85×10~4 CFU/100 ml,F(+)噬菌体浓度为0~7.70×103 PFU/L,通用拟杆菌浓度为0~6.07×10~6copies/ml,沙门氏菌检出率为30.95%,诺如病毒检出率为27.38%。统计学分析显示,粪大肠菌群与沙门菌具有相关性(r=0.243,P<0.05),与诺如病毒没有相关性;通用拟杆菌与诺如病毒具有相关性(r=0.289,P<0.01),与沙门菌没有相关性;F(+)噬菌体与沙门菌和诺如病毒均没有相关性。结论海河流域中诺如病毒与通用拟杆菌具有相关性,而与粪大肠菌群无相关性。Objective To investigate the correlation between norovirus and novel indicator microorganism in Haihe Rive.Methods In this one-year（Dec. 2013-Nov. 2014） field investigation, water samples were collected from Haihe River Basin in Tianjin. Three indicator microorganisms, fecal coliform, universal bacteroides, F（＋） bacteriophage, and two pathogenic microbes,norovirus, Salmonella, were detected in the water samples. Results The concentration of fecal coliform ranged from 0 to 6.85×10^4CFU/100 ml, the concentration of F（ ＋） bacteriophage ranged from 0 to 7. 70 × 10^3PFU/L and universal bacteroides concentration ranged from 0 to 6.07 ×10^6copies/ml. In addition, the detection rates of Salmonella and norovirus in the water samples were 30.95% and 27.38%, respectively. According to the statistical analysis, the fecal coliform was associated with the presence of Salmonella（r =0.243, P〈0.05）, but it had no correlation with the occurrence of norovirus. On the contrary, the occurrence of universal bacteroides was correlated with norovirus（r =0.289, P〈0.01）, but it was not associated with the Salmonella. The presence of F（ ＋） bacteriophage and Salmonella had no correlation with the occurrence of the norovirus.Conclusion Norovirus were correlated with universal bacteroides in Haihe River, and no correlation with fecal coliform.

关 键 词：海河 微生物污染 指示微生物 通用拟杆菌 相关性 

分 类 号：R123.2[医药卫生—环境卫生学]