山奈酚抑制顺铂诱导的大鼠心肌损伤  

Kaempferol Inhibits Cisplatin-induced Cardiac Injury in Rats

作　　者：仇炜[1] 林俊[1] 张磊[1] 朱一辰[1] 张健[1] 孙雯[1] 马麟麟[1] 田野[1] 

机构地区：[1]首都医科大学附属北京友谊医院泌尿外科,北京市100050

出　　处：《中国分子心脏病学杂志》2017年第3期2117-2119,共3页Molecular Cardiology of China

基　　金：国家自然科学基金面上项目(81372737)

摘　　要：目的山奈酚是一种有抗炎、抗氧化作用的黄酮类化合物。前期研究发现,该化合物在体外水平可抑制膀胱癌化疗药顺铂诱导的心肌细胞凋亡。本研究主要在体内条件下探讨山奈酚对顺铂心脏毒性的抑制作用。方法实验分为对照组、顺铂组、顺铂+山奈酚(50 mg/kg)组和顺铂+山奈酚(150 mg/kg)组,每组5只大鼠,采用腹腔给药处理14天。之后,分别检测心肌损伤指标LDH、CK-MB、cTnⅠ及cTnT的变化,氧化应激指标MDA、GSH和SOD的表达变化。通过ELISA检测炎症因子TNF-α的表达水平。结果在相应处理后14天,发现顺铂处理组血清LDH、CK-MB、cTnⅠ及cTnT,心脏氧化应激标志物MDA、GSH和SOD以及炎症因子TNF-α的表达水平较对照组均显著升高。而同时给予山奈酚处理的大鼠上述指标均较单纯顺铂处理组下调,且较高剂量山奈酚(150 mg/kg)组抑制作用更明显。结论山奈酚可在体内水平抑制顺铂诱导的心肌损伤。Objective Kaempferol （Kae）, a potent anti-inflammation and anti-oxidative stress flavonoid was found to exhibit antiapoptosis in cisplatin-induced cell death in cardiomyocytes in vitro. The present study was designed to investigate whether this compound show protective effects in vivo. Methods Wistar rats were randomly distributed into 4 groups （n=5 per group）： the control group （treated with vehicle）, cisplatin group, cisplatin＋Kae （50 mg/kg） and cisplatin＋Kae （150 mg/kg）. The cisplatin and Kae were treated through intraperitoneal injection for 14 days. The levels of LDti, CK-MB. cTnl and cTnT for cardiac injury, tile levels of MDA, GSH and SOD for indicating oxidative stress, and the levels of TNF-α were measured. Results Cisplatin significantly upregulated the levels of LDH, CK- MB, cTnI, cTnT, MDA, GSH, SOD and TNF-α compared with the control group. The presence of Kae antagonized the effects of cisplatin on the above factors in a dose-dependent manner. Conclusion Kae inhibits cisplatin-induced cardiac injury in vivo.

关 键 词：山奈酚 心肌损伤 顺铂 膀胱癌 

分 类 号：R737.14[医药卫生—肿瘤]