新型壳聚糖-硝酸银凝胶材料的杀菌效果及创面应用  

Effect of a novel chitosan-silver nitrate gel dressing on anti-septic and wound healing

作　　者：杨川峰[1] 彭银波[1] 郝健[1] 宋晨璐 胡艳阁[1] 姚敏[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院烧伤整形科,上海201900

出　　处：《上海交通大学学报（医学版）》2017年第7期1004-1009,共6页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的·检测新型壳聚糖–硝酸银凝胶材料的杀菌效果及其对创面愈合的影响。方法·通过使用离子半透膜检测新型凝胶材料的体外银离子释放速率;通过体外菌落计数的方法检测该材料对耐甲氧西林金黄色葡糖球菌(MRSA)、大肠埃希菌及白念珠菌的杀灭作用,利用大鼠背部的创面模型检测新材料对MRSA的杀灭作用;同时分别观察新材料、磺胺嘧啶银乳膏及莫匹罗星软膏对大鼠感染创面愈合的影响。结果·与对照组相比,新材料中的银离子可以缓慢释放;杀菌实验结果显示,较其他药物,壳聚糖–硝酸银凝胶材料具有更好的杀灭MRSA、大肠埃希菌、白念珠菌的作用;动物实验也显示新材料可以有效促进大鼠背部创面的愈合。结论·由壳聚糖和硝酸银及辅料合成的新型壳聚糖–硝酸银凝胶材料不仅具有明显的杀菌效果,同时也能够促进创面的愈合。Objective · To investigate the effects of a novel chitosan-silver nitrate gel （CSNG） dressing on anti-septic in wound healing. Methods · By using the ion membrane, the release rate of the new composite materials of silver ion was tested in vitro. Meanwhile, the anti-septic effects of CSNG on methicillin resistant Staphylococcus aureus （MRSA）, Escherichia coli and Candida albicans were tested by colony counting. The rat wound healing model was used to detect the ability of new material to kill MRSA in vivo. Results · Compared with control group, the release of silver ions of CSNG was much slower. Sterilization experiment showed that CSNG killed the MRSA, Escherichia coli and Candida albicans much more efficiently, compared to that in the other treatments. Animal experiments also showed that CSNG promoted the rats of wound healing. Conclusion · Combining chitosan with silver nitrate and supplementary material could develop a novel chitosan-silver nitrate gel material, which not only has the obvious effects on antibacterial, but also on promoting wound healing.

关 键 词：壳聚糖 硝酸银 杀菌 创面愈合 

分 类 号：R318.08[医药卫生—生物医学工程] R64[医药卫生—基础医学]