己烯雌酚、邻苯二甲酸二丁酯和丙基硫脲嘧啶对小鼠垂体瘤神经细胞增殖的联合作用研究  

Study of the joint effect of disthylstilbestrol,dibutyl phthalate and propyl thiouracil exposure on GT1-7 cells proliferation

作　　者：康静[1,2] 方海琴[2] 贾旭东[2] 宋雁[2] 刘兆平[2] 裴秋玲[1] 

机构地区：[1]山西医科大学公共卫生学院,山西太原030001 [2]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021

出　　处：《中国食品卫生杂志》2017年第3期264-268,共5页Chinese Journal of Food Hygiene

基　　金：国家自然科学基金项目(81273081)

摘　　要：目的探讨己烯雌酚(DES)、邻苯二甲酸二丁酯(DBP)、丙基硫脲嘧啶(PTU)对小鼠垂体瘤神经细胞(GT1-7神经细胞)的增殖效应及其联合作用。方法 DES的剂量为0.1、1、10、100、1 000μmol/L,DBP的剂量为0.01、1、10、100、1 000μmol/L,PTU的剂量为0.000 1、0.01、1、100、1 000μmol/L。联合作用的剂量设计为DES0.1~1 000μmol/L,DBP 0.01μmol/L,PTU 0.000 1μmol/L。采用CCK-8法检测3种物质单独作用和联合作用24 h后GT1-7神经细胞存活率。结果 DES、DBP单独作用于细胞,随DES或DBP浓度的增大细胞存活率呈现先增加后降低的趋势。PTU单独作用于细胞,各组的细胞存活率与对照组比较有所增加,但基本维持在一定水平。DES、DBP、PTU单独作用时,其浓度分别低于10、100、1 000μmol/L时,GT1-7神经细胞存活率在90%以上。DES与DBP联合暴露时,细胞存活率随DES浓度的增加而降低。DES与PTU联合暴露时,细胞存活率随DES浓度增加表现为先增加后降低,DBP与PTU联合暴露时,细胞存活率随DBP浓度增加表现为先增加后降低。与DES单独作用体系比较,0.01μmol/L DBP和0.000 1μmol/L PTU同时加入到0~10μmol/L DES后细胞存活率明显增加,差异有统计学意义(P<0.05),而DES浓度为100μmol/L时细胞存活率由80%左右降至4%左右,差异有统计学意义(P<0.05)。结论 DES、DBP、PTU对GT1-7神经细胞的增殖效应呈先促进后抑制的趋势,3种物质在一定剂量下共同作用对GT1-7神经细胞增殖呈现联合作用。Objective The single toxicity and joint toxicity of disthylstilbestrol（ DES）,dibutyl phthalate（ DBP） and propyl thiouracil（ PTU） to the proliferation of GT1-7 cells were investigated. Methods The dose of DES was 0. 1,1,10,100 and 1 000 μmol/L,the dose of DBP was 0. 01,1,10,100 and 1 000 μmol/L,and the dose of PTU was 0. 000 1,0. 01,1,100 and 1 000 μmol/L. The dose of joint toxicity were DES（ 0. 1-1 000 μmol/L）,DBP（ 0. 01 μmol/L） and PTU（ 0. 000 1μmol/L）. The cell counting Kit-8 was used to evaluate the viability of GT1-7 cells under DES,DBP and PTU alone or combined exposure. Results When DES and DBP exposed alone,the viability of GT1-7 cells increased at first and then decreased with the higher dose. Compared with the control group,the viability of GT1-7 cells exposed to PTU increased and maintained at a certain level. When the concentration of DES,DBP and PTU was below 10,100 and 1 000 μmol/L,the viability of GT1-7 cells was above 90%. When DES and DBP,DES and PTU combined exposed to GT1-7 cells,the viability was increased first and then decreased with the increase of DES concentration. When DBP and PTU combinedexposed,then cell viability had the same change. When 0. 01 μmol/L DBP and 0. 000 1 μmol/L PTU added to 0-10μmol/L DES,the cell viability increased obviously,and the difference was significant（ P 0. 05）. But when concentration increased to 100 μmol/L DES,the cell viability was decreased from 80% to 4%,and the difference was significant（ P 0. 05）. Conclusion When DES,DBP and PTU was exposed to GT1-7 cells,the cell proliferation increased at first and then decreased. When they were combined at a certain concentration,it showed a joint effect of cell proliferation on GT1-7 cells.

关 键 词：己烯雌酚 邻苯二甲酸二丁酯 丙基硫脲嘧啶 GT1-7神经细胞 联合作用 细胞毒性 神经细胞 

分 类 号：R114[医药卫生—卫生毒理学]