固定化纤维素酶提取牡丹叶黄酮的研究  

Study of Immobilized Cellulose Extraction Flavanoids from Peony Leaves

作　　者：李佩艳[1] 尹飞[2] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]河南科技大学农学院,河南洛阳471023

出　　处：《食品研究与开发》2017年第14期44-48,共5页Food Research and Development

基　　金：河南科技大学博士科研启动基金项目(4009-13480053);河南科技大学青年科学基金项目(2015QN035);农产品高值化利用与快速检测技术项目(2015XTD007)

摘　　要：以大孔树脂DM130为载体,考察给酶量、戊二醛浓度、固定化温度、固定化时间4个单因素对固定化纤维素酶提取牡丹叶黄酮的影响,通过正交试验确定固定化纤维素酶提取牡丹叶黄酮的最佳提取工艺。试验结果表明,DM130大孔吸附树脂固定化纤维素酶的最佳工艺参数为:纤维素酶浓度为1.25 m L/g固定化载体、戊二醛浓度为2%、固定化温度为50℃、固定化时间为3 h,牡丹叶黄酮提取率可达1.95%。制备的固定化酶使用4次后牡丹叶黄酮提取率仍达固定化酶第一次提取时的85.13%,说明制备的固定化纤维素酶在牡丹叶黄酮提取中具有良好的效果。DM130 macroporous resin as the carrier,the effects of cellulose amount,glutaraldehyde concentration,immobilized temperature and immobilized time on the extraction ratio of flavanoids from peony leaves were investigated.The optimum technology of extracting flavanoids from peony leaves by immobilized cellulose was determined by orthogonal experiments.The results showed that the most appropriate immobilized conditions of DM130 macroporous resin were：cellulose amount 1.25 m L/g macroporous resin,glutaraldehyde concentration2 %,immobilized temperature 50 ℃,immobilized time 3 h,and the extraction ratio of peony leaves flavanoids was 1.95 %.Immobilized cellulose could be used for 4 batches and its extraction ratio of peony leaves flavanoids was up 85.13 % of the immobilized cellulose extraction for the first time,which suggested that immobilized cellulose had a good effect on extracting total flavanoids from peony leaves.

关 键 词：纤维素酶 大孔树脂 固定化 牡丹叶 黄酮 

分 类 号：TQ28[化学工程—有机化工]