排斥性导向分子a抑制剂6FNⅢ对大鼠大脑皮质神经元缺血再灌注后自噬的作用  

Effect of repulsive guidance molecule a specific inhibitor 6FNⅢ on cortex neuron autophagy in rats after cerebral ischemia/reperfusion injury

作　　者：谢扉 秦新月[1] 张融融[1] 李敏[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,重庆400016

出　　处：《解剖学报》2017年第4期381-386,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(81271307)

摘　　要：目的探讨排斥性导向分子a(RGMa)特异性抑制剂6FNⅢ对大鼠大脑皮质神经元缺血再灌注引发的自噬及对神经功能恢复的影响。方法雄性成年SD大鼠立体定位侧脑室注射不同浓度6FNⅢ后建立大脑中动脉闭塞(MCAO)/再灌注模型,Western blotting检测RGMa下游分子脑衰反应调节蛋白-2(CRMP-2)蛋白表达水平,确定最佳6FNⅢ干预浓度。将72只雄性成年SD大鼠随机分成4组:假手术组(sham)、脑缺血再灌注组(I/R)、生理盐水干预组(I/R+NS)、6FNⅢ干预组(I/R+6FNⅢ),每组18只。缺血2h再灌注后24h,采用神经功能缺损评分评估各组大鼠神经功能恢复情况;Western blotting检测自噬相关蛋白LC3Ⅱ/Ⅰ水平;免疫荧光双标检测LC3在神经元内的表达情况;透射电子显微镜观察自噬体的形成情况。结果根据各浓度6FNⅢ干预组CRMP-2表达,确定中浓度(0.5 g/L)为本实验的最适浓度。缺血再灌注后24h,I/R组较sham组神经功能评分降低(P<0.05)、自噬相关蛋白LC3Ⅱ/Ⅰ水平升高(P<0.05)、自噬体形成增多;I/R+6FNⅢ组较I/R组神经功能评分增高(P<0.05)、自噬相关蛋白LC3Ⅱ/Ⅰ水平降低(P<0.05)、自噬体形成减少;而I/R+NS组与I/R组以上各项均无统计学意义(P>0.05)。结论 RGMa特异性抑制剂6FNⅡ可在缺血再灌注急性期抑制自噬发生,促进神经功能恢复。Objective To determine the effect of repulsive guidance molecule a（ RGMa） specific inhibitor 6FNⅢon cerebral ischemia/reperfusion（ I/R） injury-induced autophagy and neurological function. Methods Different concentrations of 6FNⅢ were injected into the right lateral ventricles of adult male Sprague-Dawley rats before I/R surgery.The most proper concentration of 6FN Ⅲ was assessed by Western blotting. The rats were randomly divided into sham operation group（ sham）,cerebral I/R group（ I/R）,I/R plus normal saline group（ I/R ＋ NS）,I/R plus 6FNⅢ intervention group（ I/R ＋ 6FNⅢ）,18 rats per group. NS and 6FNⅢ were injected into the right lateral ventricle of the rats before I/R surgery. The blood was occluded for 120 minutes,followed by 24 hours reperfusion. Neurological function was evaluated.The expression of LC3 Ⅱ/Ⅰ in the brain tissue was detected by Western blotting and immunohistochemical assay.Transmission electron microscope was used to observe the autophagosome formation. Results According to the expression of CRMP-2,the optimum concentration of 6FN Ⅲ was 0. 5 g/L. 6FN Ⅲ treatment improved neurological deficits（ P 〈0. 05）,decreased LC3 Ⅱ/Ⅰ levels（ P 〈 0. 05）,and attenuated autophagosome formation at 24 hours after cerebral I/R injury. Conclusion RGMa-specific inhibitor 6FNⅢ may protect the brain against stroke-induced injury through inhibiting autophagy.

关 键 词：排斥性导向分子a 6FNⅢ 脑缺血再灌注 自噬 免疫印迹法 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]