新型比色法快速测定天然矿泉水中的痕量汞离子  

Colorimetric detection of trace mercury in mineral water via cloud-pointextraction coupled with gold nanoparticle probe

作　　者：王正 王晓伟 薛颖[2] 赵榕[2] 罗仁才[2] 陈东 

机构地区：[1]北京市预防医学研究中心,北京100013 [2]北京市疾病预防控制中心

出　　处：《卫生研究》2017年第4期640-644,共5页Journal of Hygiene Research

基　　金：首都卫生发展科研专项(No.首发2014-3-1012)

摘　　要：目的运用浊点萃取与纳米金探针联用技术,建立快速可视化检测天然矿泉水中汞离子的方法。方法合成可与汞离子特异性结合的小粒径纳米金探针,在pH 7.5条件下,于10 mL样品中加入200μL纳米金探针溶液和400μL 5%的Triton X-114进行浊点萃取,样品中的汞离子可进入Triton X-114相生色,通过观察Triton X-114相颜色的变化以及分光光度计检测,实现天然矿泉水中痕量汞离子的定性与定量检测。结果本方法肉眼可识别的汞离子浓度最低为5μg/L,分光光度法的检出限为1.8μg/L,线性范围为0~50μg/L,相关系数0.9945,分光光度法浓度测定结果与电感耦合等离子质谱法测定结果一致。结论该方法灵敏度高,检测时间短,操作简便,可用于天然矿泉水中汞离子的快速定性以及定量检测。Objective To developed a method for rapid visual and colorimetric detection of trace high toxicity element mercury（ Hg^2＋） in natural mineral water via coupling cloud-point extraction with gold nanoparticle probe. Methods The concentration of Hg^2＋was quantified via the color degree of the Triton X-114 phase. The functionalized gold nanoparticle probe was synthesized and purified. 200 μL gold nanoparticle probe was added into 10 mL water sample（ pH 7. 5）,coupled with 400 μL,5 % Triton X-114 solution. The Hg^2＋ in water could be qualitative and quantitative analyzed according the color by naked eye or colorimetric determination. Results Under the optimized condition,the detection limit was 5 μg/L Hg^2＋in aqueous solution by naked eye. The proposed method has a linear range of 0-50 μg/L,a correlation coefficient（ r）of 0. 9945,and a detection limit of 1. 8 μg/L for colorimetric determination. The results determined by the colorimetric method were excellent agreed with the concentrations determined by inductively coupled plasma mass spectrometry method. Conclusion This result indicated that the proposed method is convenience,sensitive and can be applied in the rapid,on site detection of Hg^2＋in mineral water.

关 键 词：浊点萃取 纳米金探针 比色法 汞离子 

分 类 号：R113[医药卫生—公共卫生与预防医学] R115