血清miR-17-92基因簇对视网膜母细胞瘤诊断价值的研究  

Value of serum miR-17-92 cluster in diagnosis of retinoblastoma

作　　者：陈言钊[1] 刘智屏[1] 周克英[1] 李博[1] 

机构地区：[1]暨南大学第二临床学院暨深圳市人民医院儿科,广东深圳518020

出　　处：《中国当代儿科杂志》2017年第7期776-780,共5页Chinese Journal of Contemporary Pediatrics

基　　金：深圳市卫生计生系统科研项目(编号:20151007)

摘　　要：目的分析血清mi R-17-92基因簇对视网膜母细胞瘤(RB)的诊断价值。方法采用实时定量PCR法检测20例RB患儿、20名健康对照者的血清mi R-17-92基因簇表达水平,分析其在不同分期RB患儿的表达差异及综合治疗前后的变化,并采用ROC曲线判断其对RB的诊断价值。结果 RB患儿血清中mi R-17-92基因簇成员mi R-17-3P、mi R-17-5P、mi R-18a和mi R-20a的相对表达量均高于健康对照组,以mi R-18a升高更为显著,差异均有统计学意义(P<0.05);两组mi R-19a、mi R-19b-1和mi R-92a-1相对表达量的差异无统计学意义(P>0.05)。mi R-17-5P、mi R-17-3P、mi R-18a和mi R-20a表达量在早中期和晚期RB患儿之间的差异均无统计学意义(P>0.05),但综合治疗后表达量显著降低(P<0.05)。mi R-17-3P、mi R-17-5P、mi R-18a和mi R-20a对RB诊断价值的曲线下面积(AUC)分别为0.770、0.755、0.828和0.665,以mi R-18a的AUC最大,并具有较好的灵敏度(90%)和特异度(65%)。结论 mi R-17-92基因簇的mi R-17-3P、mi R-17-5P、mi R-18a和mi R-20a在RB患儿血清中高表达,mi R-18a有可能作为RB诊断的新指标。Objective To investigate the value of serum miR-17-92 cluster in the diagnosis of retinoblastoma （RB）. Methods Serum samples were collected from 20 children with RB and 20 healthy controls. Quantitative real-time PCR was used to measure the expression of miR-17-92 cluster. The expression of miR-17-92 cluster was compared between children with different stages of RB and the changes in the expression of miR-17-92 cluster after multimodality therapy were analyzed. The receiver operating characteristic （ROC） curve was used to investigate the value of serum miR- 17- 92 cluster in the diagnosis of RB. Results Compared with the healthy controls, the children with RB had significantly higher relative expression of miR-17-3P, miR-17-5P, miR-l 8a, and miR-20a in serum （P〈0.05）, and miR-18a showed the greatest increase. There were no significant differences in the relative expression of miR-19a, miR-19b-1, and miR- 92a-1 between children with RB and healthy controls （P〉0.05）. There were no significant differences in the expression of miR-17-5P, miR-l 7-3P, miR-18a, and miR-20a between the children with early-to-moderate stage of RB and those with advanced stage of RB （P〉0.05）, but there were significant reductions after multimodality therapy （P〈0.05）. In the diagnosis of RB, the areas under the ROC curve （AUCs） for serum miR-17-3P, miR-17-5P, miR-18a, and miR-20a were 0.770, 0.755, 0.828, and 0.665 respectively, and miR-18a had the largest AUC, with a sensitivity of 90% and a specificity of 65%. Conclusions miR-17-3P, miR-17-5P, miR-18a, and miR-20a are highly expressed in the serum of children with RB, and miR-18a may be used as a new marker for the diagnosis of RB.

关 键 词：视网膜母细胞瘤 微小核糖核酸 诊断 儿童 

分 类 号：R739.7[医药卫生—肿瘤]