白杨素增强rhsTRAIL对人胃癌SGC-7901细胞毒性  

Chrysin Enhances rhsTRAIL Induced the Cytotoxicity in Human Gastric Cancer SGC-7901 Cell Line

作　　者：韩守恒[1] 罗元红 杨小红[3] 曹建国[3] 朱建思[4] 庄英帜[1] 

机构地区：[1]南华大学第一附属医院,衡阳市421001 [2]衡阳市第一人民医院,衡阳市421001 [3]湖南师范大学医学院,长沙市410006 [4]南华大学肿瘤研究所,衡阳市421001

出　　处：《湖南师范大学学报（医学版）》2009年第3期19-21,25,共4页Journal of Hunan Normal University(Medical Sciences)

摘　　要：目的:研究白杨素(ChR)是否具有增强rhsTRAIL对人胃癌SGC-7901细胞毒性作用。方法:体外培养SGC-7901细胞。MTT比色法测定细胞毒性。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞死亡率。结果:ChR、rhsTRAIL以及两者合用对SGC-7901细胞活性的半数抑制浓度(IC50值)分别为134μmol/L、402ng/mL和47ng/mL,合并用药效应的CI值是0.4676。ChR40μmol/L、rhsTRAIL100ng/mL以及两者合用的细胞死亡率分别为4.65%±0.58%、3.60%±0.16%和49.87%±4.27%。结论:亚细胞毒性浓度的白杨素具有增强rhsTRAIL对人胃癌SGC-7901细胞毒性作用。Objective To investigate whether chrysin(ChR) enhance cytotoxicity induced by recombinant human solubility TNF-related apoptosis-inducing ligand(rhsTRAIL) in human gastric cancer SGC-7901 cell line.Methods Human gastric cancer SGC-7901 cell line was cultured in vitro.The cytotoxicity was determined using MTT assay.The cell death rate was examined by flow cytometry using PI fluorescence staining.Results IC50 of cell viability inhibition in human gastric cancer SGC-7901 cells by ChR and rhsTRAIL alone or in combination of both were 134μmol/L,402ng/mL and 47ng/mL,respectively.The CI value for ChR and rhsTRAIL was 0.4676.The death rate in human gastric cancer SGC-7901 cells by ChR(40μmol/L) or rhsTRAIL(100ng/mL) or both were 4.65%±0.58%,3.60%±0.16% and 49.87%±4.27 %,respectively.Conclusion ChR at suboptimal concentration possess augmentation of rhsTRAIL induced cytotoxicity of in human gastric cancer SGC-7901 cells.

关 键 词：胃癌 白杨素 肿瘤坏死因子相关凋亡诱导配体 细胞毒性 

分 类 号：R735.2[医药卫生—肿瘤]