芹菜素增强TRAIL诱导人宫颈癌HeLa细胞凋亡  

Potentiation of Human Cervical Cancer HeLa Cells to TRAIL-induced Apoptosis by Apigenin

作　　者：刘杰[1] 唐爱琼[1] 刘飞[2] 曹建国[2] 陈忠东[1] 

机构地区：[1]南华大学第一附属医院,湖南衡阳421001 [2]湖南师范大学医学院,湖南长沙410013

出　　处：《湖南师范大学学报（医学版）》2010年第3期5-7,共3页Journal of Hunan Normal University(Medical Sciences)

基　　金：湖南省自然科学基金(NO.03JJY5009)

摘　　要：目的:研究芹菜素(API)与重组人可溶性肿瘤坏死因子相关凋亡诱导配体(TRAIL)合用对人宫颈癌HeLa细胞凋亡的影响。方法:体外培养人宫颈癌HeLa细胞。碘化丙啶(PI)染色流式细胞术(FCM)定量分析sub-G1百分率。DNA琼脂糖凝胶电泳观察细胞DNA梯形条带。结果:API(20μmol/L)和TRAIL(20ng/mL)以及两者合用作用48h的人宫颈癌HeLa细胞sub-G1百分率分别是3.56%±0.20%、6.69%±0.40%和59.8%±4.20%;DNA琼脂糖凝胶电泳显示:20μmol/LAPI与20ng/mL的TRAIL联合处理,展示出典型DNA梯形条带图谱。结论:芹菜素具有增强TRAIL诱导HeLa细胞凋亡作用。Objective To investigate whether apigenin(API) enhances apoptosis induced by recombinant human soluble TNF-related apoptosis-inducing ligand(TRAIL) in human cervical cancer HeLa cell line.Methods Human cervical cancer HeLa cells were cultured in vitro.The percentage of the sub-G1 population was determined by flow cytometry after PI fluorescence staining.The characteristic features of cell apoptosis was examined using DNA agarose gel electrophoresis.Results Flow cytometry analysis after PI staining indicated that the percentage of the sub-G1 cell population in human cervical cancer HeLa cells by treatment with API(20μmol/ L) or TRAIL(20ng/mL) alone,and with combination for 48h were 3.56%±0.20%,6.69%±0.40%,and 59.8%± 4.20%.The ladder band could be shown in DNA agarose gel electrophoresis after co-treatment with TRAIL(20ng/ mL) and API(20μmol/L).Conclusion API possesses the effect of the enhancement of HeLa cells to TRAIL induced apoptosis.

关 键 词：宫颈癌 芹菜素 重组人可溶性肿瘤坏死因子相关凋亡诱导配体 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]