新木脂素VB-1诱导卵巢癌CoC1细胞凋亡  

Induction of apoptosis in ovarian cancer CoC1 cells by neolignan VB-1

作　　者：曾凡香[1] 周应军[2] 唐爱琼[1] 刘杰[1] 

机构地区：[1]南华大学第一附属医院妇产科,湖南衡阳421001 [2]中南大学药学院,湖南长沙410013

出　　处：《湖南师范大学学报（医学版）》2011年第1期40-42,共3页Journal of Hunan Normal University(Medical Sciences)

摘　　要：目的:研究新木脂素6-羟基-4-(4-羟基-3-甲氧基苯基)3-羟甲基-5-甲氧基-3,4-二氢(3R,4S)-2-醛基萘(VB-1)诱导人卵巢癌CoC1细胞凋亡作用。方法:体外培养CoC1细胞,碘化丙啶(PI)染色流式细胞术分析细胞凋亡率。比色法测定细胞Caspase-3活性。Western Blot分析Akt蛋白磷酸化水平。结果:VB-1显著增高CoC1细胞凋亡率和激活caspase-3(P<0.05),呈浓度依赖性。1.0μmol/L VB-1以时间依赖方式降低Akt蛋白磷酸化水平(P<0.05)。结论:VB-1能有效诱导CoC1细胞凋亡,其作用机制与抑制Akt蛋白磷酸化相关。Objective To investigate whether 6-hydroxy-4-(4-hydroxy-3-methoxyphenyl)3-hydro-methyl-7-methoxy-3,4-di-hydro-2-naphthaldehyde(VB-1),a neolignan,induces apoptosis of human ovarian cancer CoC1 cells.Methods Human ovarian cancer CoC1 cells were cultured in vitro.The apoptotic rate was examined using flow cytometry using PI fluorescence staining.Caspase-3 activity was determined using colorimetric method.The level of the phosphorylated Akt protein expression was analyzed by Western Blot.Results VB-1 significantly induced increase of apoptotic cell death and activation of caspase-3 in CoC1 cells(P<0.01),in a concentration-dependent manner.VB-1(1.0μmol/L) a time-dependent reduced the level of the phosphorylated Akt protein expression(P<0.05).Conclusion VB-1 efficiently induces on apoptosis of human ovarian cancer CoC1 cells,and its mechanisms are involved in inhibition of Akt protein phosphorylation.

关 键 词：卵巢癌 木脂素 凋亡 AKT 

分 类 号：R737.31[医药卫生—肿瘤]