白杨素敏化TRAIL诱导人胃癌SGC-7901细胞凋亡  

Sensitization of TRAIL-induced apoptosis in human gastric cancer SGC-7901 cell line by chrysin

作　　者：任翔[1] 刘飞[1] 韩守恒[2] 曹建国[1] 

机构地区：[1]湖南师范大学医学院,湖南长沙410013 [2]南华大学第一附属医院,湖南衡阳421001

出　　处：《湖南师范大学学报（医学版）》2011年第2期13-15,共3页Journal of Hunan Normal University(Medical Sciences)

基　　金：湖南省自然科学基金资助项目(03JJY5009)

摘　　要：目的:研究白杨素(ChR)是否具有增敏肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导人胃癌SGC-7901细胞凋亡作用。方法:体外培养SGC-7901细胞。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞凋亡率,琼脂糖凝胶电泳检测DNA梯形条带。结果:ChR(40μmol/L)、TRAIL(100 ng/mL)以及两者合用的细胞凋亡率分别为(4.57±0.54)%、(3.70±0.17)%和(48.81±4.11)%。两者合用后,琼脂糖凝胶电泳呈现细胞凋亡的典型DNA梯形条带。结论:白杨素具有敏化TRAIL诱导人胃癌SGC-7901细胞凋亡作用。Objective To investigate whether chrysin(ChR) sensitizes TRAIL-induced apoptosis in human gastric cancer SGC-7901 cell line.Methods Human gastric cancer SGC-7901 cell line was cultured in vitro.The cell apoptosis rate was examined by flow cytometry using PI fluorescence staining.DNA fragmentation was detected by DNA agarose gel electrophoresis.Results The apoptosis rate in human gastric cancer SGC-7901 cells treated by ChR(40μmol/L) or TRAIL(100ng/mL) or both were(4.57±0.54)%,(3.70±0.17)% and(48.81±4.11)%,respectively.The obvious fragmentation DNA was observed in combination of ChR and TRAIL.Conclusion ChR at suboptimal concentration sensitizes TRAIL-induced apoptosis in human gastric cancer SGC-7901 cells.

关 键 词：白杨素 TRAIL 胃癌 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]