DFMG对LPC诱导人脐静脉内皮细胞角蛋白18磷酸化的影响  

Effects of 7-difluoromethoxy-5,4'-dimethoxyg enistein on keratin 18 phosphorylation in HUVE-12 cells induced by lysophosphatidylcholine

作　　者：邢小伟[1] 张勇[1] 杨慧琼[1] 戴哲娟 符晓华[1] 

机构地区：[1]湖南师范大学医学院心血管病研究室,湖南长沙410006

出　　处：《湖南师范大学学报（医学版）》2012年第1期11-15,共5页Journal of Hunan Normal University(Medical Sciences)

基　　金：国家自然科学基金项目(30971270);湖南省自然科学基金重点项目(07JJ3047)

摘　　要：目的:观察7-二氟亚甲基-5,4'-二甲氧基异黄酮(DFMG)对溶血性磷脂酰胆碱(LPC)诱导人脐静脉内皮细胞(HUVE-12)角蛋白18磷酸化的影响。方法:体外培养Huve-12细胞,10μmol/L的LPC分别孵育细胞(6 h、12 h、24 h和48 h),确定LPC诱导HUVE-12细胞凋亡模型所需的最佳时间。分别用0.3、1.0、3.0μmol/L DFMG和GEN预处理HUVE-12细胞后再与LPC孵育24 h。用流式细胞术及western blotting等方法检测细胞凋亡率和角蛋白18磷酸化的表达。结果:随着LPC作用时间的延长,HUVE-12细胞凋亡率亦随之增加;DFMG和GEN可呈浓度依赖性地拮抗LPC诱导的HUVE-12角蛋白18磷酸化作用,相同浓度的DFMG作用强于GEN。结论:DFMG具有拮抗LPC诱导HUVE-12细胞角蛋白18磷酸化作用。Objective To observe the effects of 7-difluoromethoxy-5,4'-dimeth oxyg enistein on keratin 18 phosphorylation in HUVE-12 cells induced by lysophosphatidylcholine.Methods HUVE-12 cells were cultured in vitro and treated with LPC10 μmol/L for(6 h,12 h,24 h,48 h) to decide the desired time of LPC for making apoptosis model of HUVE-12 cells.HUVE-12 cells were pretreated with 0.3,1.0,3.0μmol/L of DFMG and GEN before incubation with LPC for 24h.Then apoptosis rate and the expression of keratin 18 phosphorylation are detected by flow cytometry and Western blotting methods.Results Along with the prolongation of time LPC,HUVE-12 cell apoptosis rate increased;DFMG and GEN in a concentration-dependent manner against LPC induced HUVE 12 keratin 18 phosphorylation,the same concentration of DFMG effect is better than that of GEN.Conclusion DFMG has antagonism of LPC induced by HUVE-12 cell keratin 18 phosphorylation.

关 键 词：7-二氟亚甲基-5 4-二甲氧基异黄酮 角蛋白18磷酸化 人脐静脉内皮细胞 细胞凋亡 

分 类 号：R363[医药卫生—病理学]