肝硬化患者血清PK、GSH含量检测  

The activity of Pyruvate Kinase and Glutathione in liver cirrhosis patients

作　　者：陈芊伊[1] 张志杰[1] 吕星[1] 代洪[1] 

机构地区：[1]湖南师范大学医学院检验系,湖南长沙410013

出　　处：《湖南师范大学学报（医学版）》2012年第2期20-23,27,共5页Journal of Hunan Normal University(Medical Sciences)

摘　　要：目的:检测肝硬化患者血清丙酮酸激酶(PK)、谷胱甘肽(GSH))水平,探讨两者在辅助诊断肝硬化中的变化和临床意义。方法:收集肝硬化患者的血清,分别检测血清PK、GSH含量水平。计算PK、GSH对诊断肝硬化时的阳性率、阴性率、灵敏度和特异性,并分别与丙氨酸氨基转(ALT)、总胆红素(TB)进行相关性分析。结果:肝硬化组PK活性17.17±11.97 U/L、正常组PK活性51.58±25.12 U/L,两者之间有显著性差异(P<0.001),与ALT、TB均无相关性;肝硬化组GSH含量7.23±4.56 mg/L、正常组GSH含量13.61±8.41 mg/L,两者之间有显著性差异(P<0.001),与ALT、TB均无相关性。结论:肝硬化患者血清中PK、GSH均明显低于正常对照组,PK、GSH检测对肝硬化的辅助诊断有一定价值。Objective To evaluate the activity and clinical significance of pyruvate kinase(PK) and glutathione(GSH) in liver cirrhosis patients,healthy controls,patients with hepatitis.Methods The activity of PK and GSH in liver cirrhosis patients was detected by endpoint method.The positive rate,negative rate,sensitivity and specificity of PK and GSH in the diagnosis of liver cirrhosis were calculated.And compare the correlation between PK,GSH and ALT,TB.Results The activity of PK in cirrhosis group is 17.17±11.97 U/L,that in normal group is 51.58±25.12 U/L.There had significant difference between two groups(P<0.001).PK compare with ALT,TB had no correlation.The activity of GSH in cirrhosis group is 7.23±4.56 mg/L,that in normal group13.61±8.41mg/L.There had significant difference between two groups(P<0.001).GSH compare with ALT,TB had no correlation.Conclusion The activity of PK and GSH in liver cirrhosis patients were decreased.PK and GSH can serve as reference index diagnosis liver cirrhosis.

关 键 词：肝硬化 丙酮酸激酶 谷胱甘肽 

分 类 号：R575.2[医药卫生—消化系统]