Bim介导白杨素诱导肝癌Hep 3B细胞凋亡  

Bim mediates chrysin-induced apoptosis in hepatocellular carcinoma Hep 3B cells

作　　者：戴勇[1] 周蓓[2] 肖立红[2] 曹建国[2] 廖爱军[1] 

机构地区：[1]南华大学附属第一医院消化内科,湖南衡阳421001 [2]湖南师范大学医学院,湖南长沙410013

出　　处：《湖南师范大学学报（医学版）》2012年第3期14-16,20,共4页Journal of Hunan Normal University(Medical Sciences)

基　　金：国家自然基金项目资助项目(81172375)

摘　　要：目的:研究白杨素诱导人肝癌Hep 3B细胞凋亡是否涉及上调促凋亡蛋白Bim。方法:体外培养Hep-3B细胞。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞凋亡率。细胞凋亡ELISA试剂盒检测细胞组蛋白/DNA碎片。Western blot和小干扰RNA(siRNA)转染用于探索其作用机制。结果:白杨素显著诱导Hep3B凋亡,并上调Bim表达。Bim特异性siRNA能有效拮抗白杨素诱导Hep 3B细胞凋亡作用。结论:白杨素具有诱导人肝癌Hep3B细胞凋亡作用,其作用机制涉及上调Bim表达。Objective To explore the role of proapoptotic protein Bim in chrysin-induced apoptosis of human hepatocellular carcinoma Hep 3B cells.Methods Hep 3B cells were cultured in vitro.The apoptotic cell death was examined using Flow cytometry(FCM) using propidium iodide(PI) staining and the cell apoptosis ELISA detection kit.Multiple molecular techniques,such as Western blot analysis and small interfering RNA(siRNA) transfection were used to explore the molecular mechanisms of the action.Results Treatment with chrysin caused apoptotic cell death and increases of Bim expression in Hep 3B cells.Chrysin-induced cell apoptosis were reduced by transfection with a specific siRNA targeting Bim.Conclusion Chrysin significantly induced apoptotic cell death of Hep 3B cells and its mechanism is involved in up-regulation of Bim expression.

关 键 词：肝癌 白杨素 细胞凋亡 BIM 

分 类 号：R735.7[医药卫生—肿瘤]