壮通饮对大鼠心肌缺血血瘀证内皮素1表达的影响  

Effect of Zhuangtongyin on Expression of ET-1 in Rats with Myocardial Ischemia and Blood Stasis Syndrome

作　　者：张世田[1] 黄小珊[2] 庞路路 冯悦[2] 唐汉庆[1] 黄岑汉[1] 

机构地区：[1]右江民族医学院,广西百色533000 [2]广西中医药大学,广西南宁530200

出　　处：《云南中医学院学报》2017年第3期11-14,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81460658);广西高校右江流域特色民族药研究重点实验室开放课题(kfkt2016026)

摘　　要：目的观察壮通饮对心肌缺血血瘀证大鼠血清及心肌组织内皮素1(endothelin-1,ET-1)表达的影响,探讨壮通饮对冠心病心肌缺血的保护作用机制。方法采用左冠状动脉结扎法制作大鼠心肌缺血血瘀证模型,并随机分为空白、假手术组、模型组、壮通饮低(6.8g/kg)、中(13.6g/kg)、高(27.2g/kg)剂量组、阳性对照组7个组,灌胃给药治疗4周后,分别采用酶联免疫吸附法(ELISA)和WB(Western blotting)法检测大鼠血清及心肌组织ET-1的表达。结果血清ELISA结果显示,与空白组、假手术组相比,模型组ET-1的表达明显升高(P<0.05),药物干预后,各组均可使ET-1降低,但中剂量组降低ET-1效果最明显(P<0.05)。WB检测结果显示,模型组ET-1的平均灰度值明显高于空白组、假手术组,药物干预后,各组均可使ET-1平均灰度值降低,但中剂量组降低效果最明显。结论壮通饮能减少心肌缺血血瘀证大鼠ET-1的表达,缓解血管收缩,对大鼠心肌缺血有一定的保护作用。Objective To observe the effect of Zhuangtongyin on the expression of ET-1 in serum and myocardium of rats with myocardial ischemia and blood stasis syndrome , and to explore the protective mechanism of Zhuangtongyin on myocardial ischemia in patients with coronary heart disease. Methods Rat model of myocardial ischemic blood stasis was established by left coronary artery ligation. The rats were randomly divided into blank, sham operation group, model group, Zhuangtongyin low dose （6.8g/kg）, medium（13.6g/kg）（27.2g/kg）and positive control group 7 groups. After 4 weeks of intragastric administration, the expression of ET-1 in serum and myocardium was detected by enzyme-linked immunosorbent assay （ELISA） and WB （Western blotting）. Results Serum ELISA results showed that , Compared with the blank group and the sham operation group , the expression of ET-1 in the model group was significantly increased （P〈0.05）, after treatment, ET-1 could decrease ET-1, but the effect of ET-1 was lower in middle dose group （P〈0.05）. WB results showed that, the mean gray value of ET-1 in model group was significantly higher than that in blank group, sham operation group , after the intervention , the average gray value of ET-1 was decreased , but the effect of the middle dose group was the most obvious. Conclusion Zhuangtong can reduce the expression of ET-1 in rats with myocardial ischemia and blood stasis syndrome , al eviate vasoconstriction, and protect the myocardial ischemia in rats.

关 键 词：壮通饮 心肌缺血 血瘀证 内皮素1 ENDOTHELIN 1 

分 类 号：R285.5[医药卫生—中药学]