急性淋巴细胞白血病患儿骨髓白血病细胞中microRNA-195及Bcl-2mRNA表达的意义  

Expression and significance of microRNA - 195 and Bcl - 2 mRNA in the childhood acute lymphoblastic leuke-mia cells

作　　者：张素琴[1] 王春美[1] 刘玉峰[1] 李玉琴[1] 

机构地区：[1]郑州大学第一附属医院儿科,450052

出　　处：《中华实用儿科临床杂志》2017年第15期1170-1173,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：河南省教育厅自然科学基金（2010A320028）;河南省科技计划项目（172102310079）

摘　　要：目的分析microRNA（miR）-195和Bcl-2mRNA在不同分型急性淋巴细胞白血病（ALL）患儿骨髓白血病细胞中的表达及意义。方法选择2010年7月至2016年12月在郑州大学第-附属医院住院或门诊就诊的ALL患儿为研究对象，共110例。包括初诊组、缓解组和复发组，将初诊组患儿划分为3组，分别为标危（SR）组、中危（IR）组及高危（HR）组。以同期本院行骨骼矫形手术并排除肿瘤及血液系统疾病的患儿14例为对照组。所有受试对象分别抽取骨髓样品2～5mL，应用实时荧光定量PCR方法检测上述样本中miR-195及Bcl-2mRNA的表达情况，同时通过Westernblot检测Bcl-2蛋白的表达。结果miR-195在ALL初诊及复发患儿中呈低表达现象（SR：1．25±0．15；IR：1．63±0．54；HR：0．71±0．35；复发：0．46±0．18），与对照组（4．83±0．15）比较差异均有统计学意义（t=42．44、28．63、41．35、42．65，均P=0．000），而Bcl-2mRNA在ALL初诊及复发患儿中显著高于对照组（SR：2．56±0．91；IR：4．52±0．43；HR：5．49±1．21；复发：5．32-±1．15），差异均有统计学意义（t：3．58、9．86、10．12、8．81，均P=0．000）；同时还发现，随着ALL危险程度的增高，miR-195的表达呈逐渐降低趋势，而Bcl-2mRNA的表达则逐渐升高。除此之外，Westernblot检测Bcl-2蛋白的表达亦表现出相同的结果。结论miR-195的较低表达和Bcl-2mRNA的较高表达可能成为区分ALL危险度的-个潜在指标，这对于ALL患儿的临床诊断及初诊时的危险度判断具有重要的指导意义。Objective To investigate the expression and significance of microRNA - 195 （ miR - 195 ） and Bcl- 2 in different type of childhood acute lymphoblastic leukemia （ALL） cells. Methods A total of 110 patients diagnosed as ALL from the Pediatrics Department and Outpatient Department of the First Affiliated Hospital of Zheng- zhou University from July 2010 to December 2016 were recruited. The patients included newly diagnosed group, remi- ssion group and relapse group in which the newly diagnosed group was divided into standard risk（SR） group,interme- diate risk （IR） group and high risk （HR） group. And another 14 gender- and age- matched children received bone orthopaedic surgery who excluded tumor and blood system diseases in the same hospital were recruited as control group during the same period. Bone marrow samples of 2 -5 mL were drawn from all the subjects. The samples of miR - 195 and the expression of Bcl -2 mRNA were detected by using quantitative real time polymerase chain reaction, while the expression of Bcl - 2 protein was also detected by using Western blot. Results MiR - 195 expression was lower in the newly diagnosis and relapse ALL children （ SR ： 1.25 ± 0. 15 ; IR ： 1.63 ± 0.54 ; HR ： 0.78 ± 0.35 ; relapse ： 0.46 ± O. 18 ）, compared with the control group, and there were statistically significant differences （t = 42.44,28.63,41.35, 42.65, all P = 0. 000）, but the expression of Bcl - 2 mRNA was higher in the newly diagnosis and relapse ALL children than that in the control group （ SR ：2.56 ± 0.91 ; IR ：4.52 - 0.43 ; HR ： 5.49 ± 1.28 ; relapse ：5.32 ± 1.15 ）, which had statistically significant differences （ t = 3.58,9.86,10. 12,8.81, all P = 0. 000）. With the rising of the criticality of ALL, the expression of miR - 195 reduced gradually, and the expression of Bcl -2 mRNA increased step by step. Deter- mining the protein of Bcl -2 detected by Western blot showed the same result. Conclusions The lower expression of miR - 195 and the higher expre

关 键 词：急性淋巴细胞白血病 microRNA-195 BCL-2 

分 类 号：R733.71[医药卫生—肿瘤]