栗黑褐拟层孔菌胞外多糖的提取纯化、结构表征及免疫调控研究  

Extraction, purification, structure characterization and immune regulation of extracellular polysaccharide from Fomitopsis castaneus imaz

作　　者：郭文奎[1,2] 池玉杰[2] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150036 [2]东北林业大学林学院,哈尔滨150040

出　　处：《食品科技》2017年第7期190-194,共5页Food Science and Technology

摘　　要：利用DEAE-Sepharose fast flow色谱层析柱及真空冻干技术对栗黑褐拟层孔菌胞外多糖进行分离纯化,并利用苯酚-硫酸法、红外光谱、高效液相色谱等化学分析手段对主要多糖进行结构表征;通过构建小鼠模型,对小鼠脏器系数进行分析,提取回肠及空肠组织RNA,采用Real-time RT-PCR方法对免疫相关基因IL-4、β-actin、IFN-γ和TNF-α的表达情况进行分析。研究表明:栗黑褐拟层孔菌菌丝体内多糖主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖及半乳糖组成,其摩尔比为43.40:0.09:0.13:51.32:5.06;高效液相色谱计算主要多糖分子量为3.24×104 u,红外光谱分析表明栗黑褐拟层孔菌胞外多糖具有多糖类物质的结构特征;相关免疫脏器系数随着天数的增加而增加,实验组的脏器系数较对照组相比,显著增加;相关免疫调控基因IL-4、β-actin、IFN-γ和TNF-α的表达量随着时间的延长均有所增加。The DEAE-Sepharose fast flow chromatography column and vacuum freeze-drying technique were used to separate and purify the extracellular polysaccharide from Fomitopsis castaneus imaz, and analyzed by phenol-sulfuric acid method, infrared spectroscopy and high performance liquid chromatography. The expression of IL-4, β-actin, TNF-aand IFN-ywere detected by real-time RT-PCR. The results showed that the EPS was mainly composed of glucose, galactose, rhamnose, mannose, and arabinose at a molar ratio of 43.40：0.09：0.13：51.32：5.06. The molecular weight of the polysaccharide was about 3.24×104 u. Moreover, the characteristic absorption peaks of sugar esters were detected. The number of related immune organs increased with the number of days, the organ coefficient of the experimental group was significantly higher than that of the control groups. The expression of IL-4, β-actin, IFN-y and TNF-a increased with the prolongation of time.

关 键 词：多糖 表征 纯化 免疫调控 

分 类 号：R284[医药卫生—中药学]