小鼠肉瘤细胞分泌的生长因子对裸鼠坐骨神经损伤修复的探讨  

The effects of nerve growth factor S180 on repairing sciatic nerve in mice

作　　者：马建东[1] 殷刚 李强[1] 

机构地区：[1]宁夏银川市第一人民医院,宁夏银川750001 [2]宁夏银川市紧急救援中心,宁夏银川750001

出　　处：《宁夏医学杂志》2017年第7期580-581,共2页Ningxia Medical Journal

摘　　要：目的探讨小鼠肉瘤细胞S180分泌的神经生长因子(Nerve Growth Factor,NGF)与外源性神经生长因子对损伤坐骨神经的修复效果。方法将裸鼠分为8组,实验操作后观察其肢体运动恢复情况,采用改良Tarlov评分,取血液标本检测NGF浓度;取脊髓腰膨大处做切片染色,观察受体Trka和p75。结果小鼠肉瘤细胞S180分泌NGF,且接种量不同,NGF产生量也不同,接种1×10~4为0.390±0.018,接种1×10~5为0.445±0.012,接种2×10~5为0.469±0.017,接种1×10~6为0.520±0.049,接种2×10~6为0.571±0.042;接种组神经损伤恢复效果明显优于未接种组;改良评分接种组接种1×10~4为2.00±0.00,接种1×10~5为2.25±0.50,接种2×10~5为2.75±0.50,接种1×10~6为2.75±0.50,接种2×10~6为2.50±0.57,均优于其他组;切片显示接种组Trka和P75均表达增高。结论接种肉瘤细胞S180可产生内源性NGF,内源性NGF对周围神经损伤修复效果优于外源性NGF。Objective To study the repair function of nerve growth factor （NGF） which secrete by sarcomas 180 and extrinsic nerve growth factor on sciatic nerve. Methods Rats were randomly divided into eight groups, included five groups, NGF group, normal group and four rats in each group ,except normal group. Others were cut sciatic nerve and anastomosed, and 6bserved the hindlimb func- tion, NGF concentration, Trka and p75. Results Sarcomas 180 secreted nerve growth factor, also the concentration of nerve growth factor were different, the rats inoculated group were better recovery than those in other groups. The Trka and p75 which rats inoculated had ele- vation than those in other groups. Neurotrophin receptors Trka and p75. Conclusion Rats inoculated sarcomas can secrete endogenous nerve growth factor, it has a good effects than extrinsic nerve growth factor.

关 键 词：肉瘤细胞S180 神经生长因子 坐骨神经损伤 

分 类 号：R745[医药卫生—神经病学与精神病学]