宫颈癌组织中maspin、AKT、p-AKT表达变化及其与肿瘤微淋巴管密度的相关性  

Expression of maspin,AKT,and p-AKT in cervical carcinoma and its correlation with tumor lymphatic microvessel density

作　　者：许振[1] 刘志强[1] 孟玮[1] 郭庆枝[1] 孙莉[1] 

机构地区：[1]滨州医学院附属医院,山东滨州256600

出　　处：《山东医药》2017年第27期23-26,共4页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(ZR2012HL01)

摘　　要：目的观察宫颈癌组织中maspin、蛋白激酶B(AKT)、磷酸化蛋白激酶B(p-AKT)的表达变化,并分析其与肿瘤微淋巴管密度(LMVD)的相关性。方法收集127例份宫颈石蜡组织标本,其中慢性宫颈炎组织20例份(炎症组)、CINⅠ级组织20例份(CIN1组)、CINⅡ级组织20例份(CIN2组)、CINⅢ级组织20例份(CIN3组)、宫颈癌组织47例份(宫颈癌组,其中无淋巴结转移32例为宫颈癌1组、有淋巴结转移15例为宫颈癌2组)。采用免疫组化SP法检测各组maspin、AKT、P-AKT,测算肿瘤微淋巴管密度(LMVD)。结果宫颈癌1组、宫颈癌2组maspin蛋白阳性表达比例低于其余各组(P均<0.05)。宫颈癌1组、宫颈癌2组AKT、p-AKT阳性表达比例高于其余三组(P均<0.05)。宫颈癌2组p-AKT阳性表达比例高于宫颈癌1组(P<0.05)。宫颈癌组织中maspin与AKT表达呈负相关关系(r_s=-0.472,P=0.001),maspin与p-AKT表达也呈负相关关系(r_s=-0.598,P=0.000)。宫颈癌1组、宫颈癌2组LMVD分别为(19.44±2.95)、(23.24±2.46)支/mm^2,淋巴结转移与LMVD呈正相关关系(r_s=0.44,P=0.000)。宫颈癌组织中LMVD与maspin表达呈负相关关系(r_s=-0.554,P=0.000),LMVD与p-AKT表达呈正相关关系(r_s=0.39,P=0.007)。结论宫颈癌组织中maspin表达下调,AKT、p-AKT表达上调,且maspin表达与AKT、p-AKT表达均呈负相关关系;宫颈癌组织中LMVD与maspin表达呈负相关关系,LMVD与p-AKT表达呈正相关关系。maspin表达下调可能参与了宫颈癌的发生发展,机制可能与AKT通路异常激活、促进肿瘤微淋巴管生成有关。Objective To observe the expression changes of maspin,protein kinase B （AKT）,and phosphorylated protein kinase B （p-AKT） in the cervical cancer tissues and to analyze its correlation with tumor lymphatic microvessel density （LMVD）.Methods Twenty-seven cases of cervical paraffin tissues were collected,including 20 cases of chronic cervicitis （inflammation group）,20 cases of cervical intraepithelial neoplasia （CIN） Ⅰ （CIN1 group）,20 cases of CIN Ⅱ （CIN2 group）,20 cases of CIN Ⅲ （CIN3 group）,and 47 cases of cervical cancer tissues （cervical cancer group,including 32 cases without lymph node metastasis of cervical cancer group 1 and 15 cases with lymph node metastasis of cervical cancer group 2）.The levels of maspin,AKT,and P-AKT were measured by immunohistochemical SP method,and the LMVD was measured.Results The positive rate of maspin protein in the cervical cancer group 1 and cervical cancer group 2 was lower than that in the CIN3 group （all P〈0.05）.The positive expression rates of AKT and p-AKT in the cervical cancer group 1 and cervical cancer group 2 were higher than those in the other three groups （all P〈0.05）.The positive expression rate of p-AKT in the cervical cancer group 1 was higher than that in the cervical cancer group 2 （P〈0.05）.There was a negative correlation between the expression of maspin and AKT in the cervical cancer tissues （rs=-0.472,P=0.001）.There was also a negative correlation between maspin and p-AKT expression （rs=-0.598,P=0.000）.LMVD in the cervical cancer groups 1 and 2 were （19.44±2.95） and （23.24±2.46） vessels/mm^2,respectively.There was a positive correlation between lymph node metastasis and LMVD （rs=0.44,P=0.000）.There was a negative correlation between LMVD and the expression of maspin in the cervical cancer tissues （rs=-0.554,P=0.000）.There was a positive correlation between LMVD and p-AKT expression （rs=0.39,P=0.007）.Conclusions The expression of AKP and p-AKT is up-regulated in the cervic

关 键 词：宫颈癌 MASPIN基因 蛋白激酶B 微淋巴管密度 

分 类 号：R737.33[医药卫生—肿瘤]