姜提取物生物合成纳米银抑菌活性的研究  

Antibacterial Study of Silver Nanoparticles Biosynthesized with Ginger Extract

作　　者：刘冲冲 王磊[2] 徐慧[1] 姜宇[2] 李福艳[2] 宿红艳[3] 

机构地区：[1]鲁东大学化学与材料科学学院,山东烟台264025 [2]鲁东大学生命科学学院,山东烟台264025 [3]鲁东大学农学院,山东烟台264025

出　　处：《食品与生物技术学报》2017年第6期590-597,共8页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31570649);山东省自然科学基金项目(ZR2013CM018);山东省高校科技项目(J15LE03);烟台市科技发展计划项目(2013NC049)

摘　　要：作者利用姜提取液作为还原剂生物合成具有抑菌活性的纳米银材料,避免了传统合成过程中危害环境的弊端。生物合成的纳米银材料分别采用紫外吸收光谱(UV-vis)、透射电子显微镜(TEM)、X射线晶体衍射(XRD)等方法进行表征。结果表明,该纳米银颗粒为面心立方结构,呈球形,粒径范围在5~30 nm,分散性良好。体外抑菌结果显示,姜提取液合成的纳米银对供试的8种典型病原菌(包括4种典型的临床耐药病原菌和4种海洋水产病原菌)均具有显著的抑菌效果,对金黄色葡萄球菌的最小抑菌浓度和最小杀菌质量浓度分别为25.7μg/m L和51.4μg/m L。长期稳定性实验和热稳定性实验表明,该生物合成的纳米银具有优良的稳定性。最终实验结果表明,利用姜提取物能够合成出环境友好、广谱高效、性能稳定的新型抗菌剂。Ginger（Zingiber officincde Roscoe） extract was found to have a brand new application in this paper. The antibacterial silver nanoparticles （AgNPs） were synthesized using ginger extract as a reducing agent, which avoid the disadvantages of environmental hazards in traditional methods. The as-prepared AgNPs were fully characterized by means of UV-visible （UV-vis） spectroscopy, transmission electron microscopy （TEM） and X-ray diffraction （XRD） technique. The results showed that the AgNPs was face-centered cubic structure,and the spherical-shaped nanoparticles were polydisperse in the size range 5-30 nm. The synthesized silver nanomaterials showed remarkable antibacterial activity against all tested pathogens including four kinds of multidrug resistant clinical isolates and four kinds of aquatic pathogens. The minimum inhibitory concentration（MIC） and the minimum bactericidal concentration （MBC） of the AgNPs against Staphylococcus aureus were 25.7 μg/mL and 51.4 μg/mL, respectively. Moreover, stability studies showed that the biosynthesized AgNPs had excellent long-term stability as well as thermal stability. Therefore, the ginger extract could be used to synthesize a new kind of AgNPs with stable and broad-spectrum antimicrobial activity.

关 键 词：姜 纳米银 生物合成 抑菌活性 稳定性 

分 类 号：TQ465[化学工程—制药化工]