hTERC基因在预测宫颈上皮内瘤变Ⅰ级进展和转归中的价值  

The Value of hTERC Gene in Predicting the Progression and Outcome of GradeⅠCervical Intraepithelial Neoplasia

作　　者：葛运贞 

机构地区：[1]山东省临沂市经济技术开发区人民医院,山东临沂276023

出　　处：《中国医学创新》2017年第21期31-34,共4页Medical Innovation of China

摘　　要：目的:探讨hTERC基因在预测宫颈上皮内瘤变Ⅰ级进展和转归中的价值,为临床预测宫颈上皮内瘤变Ⅰ级进展和转归提供理论依据。方法:选取本院2011年12月-2016年12月收治的206例宫颈上皮内瘤变Ⅰ级(CINⅠ)进行分析,随访后根据病理学确诊情况分为进展组、持续组和转归组,采用Fish技术检测不同组的hTERC基因扩增情况。结果:206例宫颈石蜡包埋病理标本,所有患者均成功杂交,其中56例进行了二次杂交,杂交成功率100%。206例CINⅠ组患者随访后发现,hTERC基因扩增阳性114例,其中进展组67例、持续组30例、转归组17例。在进展组中hTERC基因扩增阳性67例,占100%,持续组中基因扩增阳性30例,占49.18%,转归组中基因扩增阳性17例,占21.79%;经Fisher确切概率法,三组转归阳性率相比,差异有统计学意义(P<0.05)。经Fisher确切概率法,三组hTERC基因扩增异常信号比的细胞比例比较,差异有统计学意义(P<0.05)。结论:hTERC基因或可成为预测CINⅠ病程进展程度的一种生物学指标,可为临床医师诊断和治疗提供依据,从而更大程度地做到对患者的早诊、早治,避免对患者造成更大的伤害。Objective： To explore the value of hTERC gene in predicting the progression and outcome of cervical intraepitbelial neoplasia grade I, and to provide theoretical basis for predicting the progression and outcome of cervical intraepithelial neoplasia grade I.Method： A total of 206 cases of cervical intraepithelial neoplasia （CINI）were selected in our hospital from December 2011 to December 2016.The patients were divided into progress group, continuous group and control group, according to the pathologic diagnosis, Fish technique was used to detect the hTERC gene amplification in different groups.Result： 206 cases of cervical paraffin embedded pathological specimens, all patients were successfully hybridized, of which 56 cases were secondary hybridization, hybridization success rate of 100%.206 patients with CIN I were followed up and found that 114 cases of hTERC gene amplification were positive, including 67 cases in the progress group, 30 cases in the continuous group and 17 cases in the control group.In the progress group, the positive rate of hTERC gene amplification was 67 cases （ 100% ）, the continuous group was positive in 30 cases （ 49.18% ）, and the control group was 17 cases （ 21.79% ）, by Fisher exact test method, the difference between the three groups was statistically significant （P〈0.05） .The Fisher exact test, the proportion of cells in the abnormal signal amplification of hTERC gene than the three groups compared, the difference was statistically significant （ P〈0.05 ） . Conclusion： The hTERC gene may be a biological indicator for predicting the progression of CIN I, which can provide a basis for the diagnosis and treatment of clinicians, so as to achieve a greater degree of early diagnosis and early treatment of patients, to avoid causing greater harm to patients.

关 键 词：宫颈癌 宫颈上皮内瘤变 人类染色体端粒酶RNA基因 荧光原位杂交技术 石蜡包埋切片 

分 类 号：R737.33[医药卫生—肿瘤]