艾灸对亚急性衰老大鼠胸腺CD4^+T细胞IL-2、IL-2Rα、NF-κB和I-κB蛋白表达的影响  

Effects of Moxibustion on the Expression of IL-2,IL-2Rα,NF-κB and I-κB in Thymus CD4^+T Cells of Subacute Aging Rats

作　　者：崔云华[1] 沈文宾 吴焕淦[1] 周次利[1] 雷菲[1] 黄立师 赵琛[2] 李璟[3] 

机构地区：[1]上海中医药大学上海市针灸经络研究所,上海200030 [2]上海中医药大学,上海201203 [3]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《世界中医药》2017年第5期1094-1100,1104,共8页World Chinese Medicine

基　　金：国家重点基础研究发展计划("973"计划)资助项目(编号:2009CB522900;2015CB554501);国家自然科学基金项目(编号:81303029;81373752)

摘　　要：目的:通过观察IL-2及相关受体蛋白水平的变化情况,探讨艾灸对亚急性衰老大鼠胸腺CD4+T细胞的影响。方法:采用D-半乳糖致亚急性衰老大鼠模型。24只清洁级雄性SD大鼠随机分为:正常组,模型组和艾灸组。艾灸组温和灸双侧肾俞穴5 min/次,共计40 d。采用免疫磁珠法分离胸腺CD4+T细胞,并再用Western Blot方法检测各组大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB的蛋白表达量。结果:模型组大鼠胸腺CD4+T细胞中IL-2、IL-2Rα、NF-κB、I-κB蛋白的表达量较正常组降低,差异有统计学意义(P<0.05);艾灸组大鼠胸腺CD4+T细胞中IL-2、IL-2Rα、NF-κB、I-κB蛋白表达量升高,与模型组比较差异有统计学意义(P<0.05)。结论:艾灸肾俞穴可上调胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB、I-κB的表达。Objective：To observe the effect of moxibustion on CD4＋T cells of thymus in subacute aging rats by recording the changes of IL-2 and related receptor protein levels.Methods：Use D-galactose induced subacute aging rat model.Twenty-four male SD rats were randomly divided into normal group, model group and moxibustion group.In moxibustion group, use mild moxibustion on Shenshu Point（BL23） bilateral for 40 days, 5 minutes each day each time.To separate CD4＋T cells by MACS method, and access the expression of IL-2, IL-2Rα, NF-κB and I-κB in CD4＋T cell by Western Blot.Results：Western Blot assay shows that comparing to the control group, the expression of IL-2, IL-2Rα, NF-κB and I-κB in CD4＋T cell of model group significantly decreased （P〈0.05）.However, in the moxibustion group, those increased with significant difference （P〈0.05）.Conclusion：In subacute aging rats, moxibustion on BL23 can improve the expression of IL-2, IL-2Rα, NF-κB and I-κB in thymic CD4＋T cell.

关 键 词：亚急性衰老 IL-2 IL-2RΑ NF-ΚB I-ΚB 艾灸 

分 类 号：R245.81[医药卫生—针灸推拿学]