福建省53份茶树种质资源遗传多样性ISSR分析  

Analysis of Genetic Diversity of Fifty-three Camellia Sinensis in Fujian Province by ISSR

作　　者：朱晨[1] 张舒婷[1,2] 常笑君[1] 王仲[1] 许长同 张梓浩[1,2] 林玉玲[1,2] 赖钟雄[1,2] 郭玉琼[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺植物生物工程研究所,福建福州350002 [3]福州市农业局,福建福州350001

出　　处：《热带作物学报》2017年第7期1303-1310,共8页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金(No.2013J01085);福建省重大科技专项(No.2015NZ0002-1);福建省教育厅计划项目(No.JA15143)资助

摘　　要：利用ISSR分子标记技术对福建省7个地区的茶树品种进行遗传多样性和遗传结构的分析。筛选出的12条引物共扩增出97条条带,其中多态性条带有92条,多态性谱带百分率为94.84%;53份供试材料可划分为5大类(GS=0.71),遗传距离介于0.66~0.99之间;Nei基因多样性指数为0.224,Shannon's信息指数为0.368,不同地区茶树的遗传多样性参数差异较大,其中宁德福安地区最高,福州鼓山地区最低;总变异系数为0.332,地区内个体间遗传多样性为0.154,而地区间为0.231,福州地区与其他地区之间的相似性较弱;研究认为,福建省茶树遗传多样性丰富,不同地区茶树存在高频率遗传变异,而缺乏遗传信息交流是造成福州地区遗传多样性较低的可能因素。The genetic diversity and genetic structure of tea plant in seven regions of Fujian Province were examined by ISSR.The results showed that, 97 bands were amplified with 12 ISSR primers, the polymorphic bands of which were 92, and their polymorphism percentage was 94.84%. 53 tea plant resources were divided into 5groups（GS=0.71）. The range of genetic distance was between 0.66~0.99. The Nei＇ s genetic distance was 0.224,the Shannon＇ s information index was 0.368, and the genetic diversity in different regions of tea plant had significant？difference. Fu＇an in Ningde was the highest when the Gushan Mountain in Fuzhou got the lowest similarity with the others. The coefficient of gene variation was 0.332. The values of genetic diversity in and between the regions was 0.154 and 0.231, respectively.The Fuzhou area got low similarity with the others. The results indicated that, there was high genetic diversity of tea plant in Fujian Province, which had high frequency of heritable variation in different regions. The insufficiency of genetic information flow might be the main fact of the genetic diversity in Fuzhou area.

关 键 词：茶树 种质资源 ISSR 遗传多样性 

分 类 号：S571.1[农业科学—茶叶生产加工]