维生素C碳点对口腔鳞状细胞癌KB细胞增殖、自噬和凋亡的影响  

Influence of vitamin C carbon dots in proliferation,autophagy and apoptosis of oral squamous cell carcinoma KB cells

作　　者：唐琪[1] 王丹丹[1] 布文奂[1] 李杏[1] 王璐[1] 孟琳[1] 孙宏晨[1] 

机构地区：[1]吉林大学口腔医院病理科吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2017年第4期667-671,I0001,共6页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(8132010801;81271111);吉林大学研究生创新基金资助课题(2016215;2016112)

摘　　要：目的:研究维生素C碳点对口腔黏膜鳞状细胞癌(口腔鳞癌)KB细胞的杀伤作用,探讨其相关作用机制。方法:以不同浓度(5、10、20、40和80mg·L^(-1))维生素C碳点体外处理口腔鳞癌KB细胞作为实验组,以0mg·L^(-1)维生素C碳点组作为空白对照组。MTT法检测各组细胞增殖率,克隆形成实验检测各组细胞克隆形成能力,Western blotting法检测各组细胞中自噬相关蛋白LC3蛋白表达水平,流式细胞术检测KB细胞的凋亡率。结果:与空白对照组比较,20、40和80mg·L^(-1)维生素C碳点组KB细胞的增殖率及克隆形成能力均明显降低(P<0.01),40mg·L^(-1)维生素C碳点组KB细胞中LC3Ⅱ蛋白表达水平和细胞凋亡率明显升高(P<0.05或P<0.01)。结论:维生素C碳点能够有效地杀伤口腔鳞癌KB细胞,抑制KB细胞的增殖并减弱其克隆形成能力,其杀伤作用可能与KB细胞自噬和凋亡的发生有关。Objective： To study the lethal effect of vitamin C carbon dots on oral squamous cell carcinoma KB cells, and to clarity its related mechanism. Methods： The KB cells were treated with different concentrations （5, 10, 20, 40 and 80 mg· L^-1） of vitamin C carbon dots in vitro as experimental groups, and 0 mg · L^-1 vitamin C carbon dots group was used as blank control group. MTT assay was used to detect the proliferation rates of KB cells in various groups; colony formation assay was used to detect the colony formation ability of KB cells; Western blotting was performed to detect the protein expression levels of autophagy related protein LC3 in KB cells in various groups ; flow cytometry was used to detect the apoptotic rates of KB cells in various groups. Results： Compared with blank control group, the proliferation rates and colony formation abilities of KB cells in 20, 40 and 80 mg· L^-1 carbon dots groups were markedly decreased （P 〈 0.01）. Compared with blank control group, the protein expression level of LC3 II in 40 mg · L^-l carbon dots group was increased （P〈0.05） ; the apoptotic rate of KB cells was markedly increased （P〈0.01）. Conclusion： Vitamin C carbon dots can kill the oral squamous cell carcinoma KB cells effectively, suppress the proliferation and impair the colony formation ability of KB cells, which is related to autophagy and apoptosis of KB cells.

关 键 词：维生素C碳点 口腔黏膜 癌 鳞状细胞 增殖 自噬 细胞凋亡 

分 类 号：R739.85[医药卫生—肿瘤]