尿素通道蛋白B在人膀胱癌组织中的表达及其临床意义  

Expression of urea transporter B in human bladder cancer tissue and its clinical significance

作　　者：历春[1] 付天悦 杨宝学[3] 盖晓东[1] 

机构地区：[1]北华大学基础医学院免疫学教研室,吉林吉林132013 [2]吉林大学药学院 [3]北京大学基础医学院药理学系,北京100871

出　　处：《吉林大学学报（医学版）》2017年第4期705-708,I0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基础研究计划项目资助课题(20150101128JC);吉林省教育厅项目资助课题(2013193)

摘　　要：目的:检测膀胱癌患者肿瘤组织和正常膀胱黏膜组织中尿素通道蛋白B(UT-B)的表达,阐明其在膀胱癌组织中表达的临床意义。方法:收集临床膀胱癌患者术后石蜡包埋标本52例,以正常膀胱黏膜组织15例作为对照,采用免疫组织化学方法检测UT-B蛋白阳性表达率,分析UT-B蛋白阳性表达率在膀胱癌组织和正常膀胱黏膜表达的差异以及其与患者临床病理参数的关联。结果:UT-B蛋白在膀胱癌组织中低表达,阳性表达率为44.2%;在正常膀胱黏膜组织中高表达,阳性表达率为93.3%,2组UT-B蛋白阳性表达率比较差异有统计学意义(P=0.001)。膀胱癌组织中UT-B蛋白的阳性表达率随肿瘤组织分级的增高而逐渐降低,不同分级间比较差异有统计学意义(P=0.010)。膀胱癌组织中UT-B蛋白在非肌层浸润期(Ta+T1)的阳性表达率明显高于肌层浸润期(T2+T3),组间比较差异有统计学意义(P=0.014)。结论:UT-B蛋白的表达降低或缺失可能促进了膀胱癌的发生发展和侵袭。Objective： To detect the expressions of urea transporter B （UT-B） in tumor tissue of bladder cancer patients and normal urothelium tissue, and to investigate its clinical significance of expression in bladder cancer tissue. Methods： Fifty-two paraffin-embedded specimens of bladder cancer patients and 15 normal urothelium specimens were selected. The expression levels of UT-B protein were detected by immunohistochemistry method. The difference of expression of UT-B in bladder cancer tissue and normal urothelium tissue and its relationship with the clinicopathological parameters were analyzed. Results： The expression rate of UT-B protein in bladder cancer tissue was 44.2%, while it was significantly higher in control group （93.3%）, and the difference between two groups was significant （P= 0. 001）. The positive expression rates of UT-B in bladder cancer tissue were significantly decreased with the increasing of histological grades, and there were significant differences between different grade groups （P=0. 010）. The positive expression rate of UT-B in muscle-invasive stage （Ta＋T1） was significantly lower than that in non-muscle-invasive stage （T2＋T3） （P=0. 014）. Conclusion： The reduction or lack of UT-B expression may oromote the incidence, progression and invasiveness of bladder cancer.

关 键 词：膀胱肿瘤 尿素通道蛋白B 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]