逍遥散调节慢性应激肝郁脾虚模型大鼠下丘脑弓状核ob-R、α-MSH变化机制  

Mechanism of Xiaoyaosan Decoction on ob-R and α-MSH in arcuate nucleus of hypothalamus of chronic restraint stress modeling rats with liver depression and spleen deficiency

作　　者：旷湘楠 王少贤[1] 方朝义[1] 王杰鹏[1] 陈家旭[2] 刘玥芸[2] 

机构地区：[1]河北中医学院,石家庄050200 [2]北京中医药大学基础医学院,北京100029

出　　处：《世界中医药》2017年第3期488-493,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81202644;81673881;81630104);河北省自然科学基金项目(编号:H2016423049);高等学校博士学科点专项科研基金资助课题(编号:20121323120016);河北省教育厅高等学校科学研究项目(编号:QN2014118)

摘　　要：目的:研究中药复方逍遥散(《太平惠民和剂局方》)疏肝解郁、健脾以调节食欲的作用机制。方法:以每天持续3 h慢性束缚应激方法制备动物模型。SD健康雄性大鼠随机分为正常组、7 d模型组、21 d模型组和逍遥散组4组,正常组予常规饲养,模型组和逍遥散组每天选择随机时间点进行束缚应激,7 d模型组束缚应激7 d,21 d模型组束缚应激21 d,逍遥散组在连续束缚应激21 d同时予逍遥散灌胃。观测各组大鼠体重、摄食量、行为学、血清D-木糖含量变化;免疫荧光双染、RT-q PCR方法观测下丘脑ARC中瘦素受体(Leptin Receptor,ob-R)、α-促黑素细胞激素(α-melanocyte Stimulating Hormone,α-MSH)基因与蛋白表达水平。结果:与正常组比较,束缚应激21 d模型大鼠体重、摄食量和血清D-木糖含量明显降低,行为学实验表明模型大鼠自主活动能力下降、对新异环境探究行为明显减少,而逍遥散则具有相应的调节作用。综合表明,每天3 h连续21 d捆绑束缚可制备慢性应激肝郁脾虚样动物模型,慢性束缚应激大鼠下丘脑弓状核(Arcuate Nucleus,ARC)中ob-R、α-MSH表达水平增加,逍遥散可下调其表达。结论:逍遥散调节下丘脑ARC中ob-R和α-MSH表达变化,可作为其疏肝健脾,调节机体食欲和能量代谢影响脾胃功能的作用靶点之一。Objective：To study the mechanism of Xiaoyaosan Decoction in regulating appetite by soothing liver to relieve depres- sion, and invigorating spleen. Methods ： SD male rats were randomly divided into four groups ; the control group （ A ） , the 7-day model group （B） , the 21-day model group （C） and the Xiaoyaosan （XYS） group （D）. Except for the control group, models of the other three groups were established by chronic restraint stress method 3 h a day persistently for 7 days, 21 days and 21 days re- spectively, while Xiaoyaosan Decoction was given in the XYS group at the same time. Body weight, food intake, animal behavior, and the level of D-xylose in the serum were detected in all four groups ; the mRNA and protein levels of leptin receptor（ob-R） and- melanocyte stimulating hormone（-MSH） were measured by RT-qPCR and immunofluorescence double staining method. Results： Compared with the control group, the body weight, food intake and the level of D-xylose in the serum were significantly decreased in group C, with decreased autonomous activity and new environment exploratory behavior ; while the result of group D showed that Xiaoyaosan Decoction could regulate all of those mentioned above. All together indicated that chronic restraint animal model with liver depression and spleen deficiency can be established by restraining 3 h a day, for 21 days continually; the expression of ob-R and-MSH increased in arcuate nucleus （ARC） of chronic restraint stress rats and Xiaoyaosan Decoction could reduce the expres- sion. Conclusion ： The expression of ob-R and-MSH can be regulated by Xiaoyaosan Decoction, indicating that ob-R and-MSH may be the targets of Xiaoyaosan Deeoction in regulating the function of spleen and stomach through soothing the liver and invigorating the spleen so as to regulate appetite and energy metabolism.

关 键 词：慢性应激 逍遥散 肝郁脾虚 瘦素受体 Α-促黑素细胞激素 

分 类 号：R228[医药卫生—中医基础理论]