慢性束缚应激抑郁症大鼠海马Belin-1、LC3的表达变化及逍遥散的调节作用  

Effects of Xiaoyaosan Decoction on the expression of Belin-1 and LC3 inhippocampus of rats with depression induced by chronic restraint stress

作　　者：王亭晔 李晓娟[1] 周雪明[1] 潘秋霞[1] 陈家旭[1] 

机构地区：[1]北京中医药大学中医学院,北京100029

出　　处：《世界中医药》2017年第3期509-512,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81473597;81630104);北京市自然科学基金项目(编号7152093)

摘　　要：目的:观察慢性束缚应激抑郁症大鼠模型中海马自噬标志蛋白Belin-1及LC3的表达变化情况,探讨逍遥散对其的调节作用。方法:20只Wistar大鼠随机分为4组(n=5):正常组、模型组、逍遥散组、氟西汀组。采用21 d慢性束缚应激方法建立抑郁症大鼠模型,利用免疫组织化学方法检测大鼠海马CA3区Belin-1、LC3表达情况。结果:Belin-1、LC3在各组中较正常组均有较明显的表达(P<0.05)。与模型组相比,逍遥散能明显下调Belin-1、LC3的表达(P<0.05)。结论:慢性束缚应激抑郁症大鼠海马自噬相关蛋白Belin-1、LC3表达有变化,提示自噬可能是抑郁症发病机制之一,且逍遥散治疗抑郁症的机制可能与调节自噬标志蛋白Belin-1、LC3有关。Objective： To observe the effects of Xiaoyaosan Decoction on the expression of Belin-1 and LC3 in hippocampus of rats with depression induced by chronic restraint stress. Methods： A total of 20 Wistar rats were randomly divided into 4 groups ： the control group, the model group, the Xiaoyaosan （XYS） group and the fluoxetine group. Depression model was established by 21- day chronic restraint stress, and the expression of Belin-1 and LC3 in CA3 of rats＇ hippocampus was detected by immunohisto- chemistry. Results ： There is significant differences between the control group and other groups （ P 〈 0. 05 ）. Xiaoyaosan Decoction could down-regulated the expression of Belin-1 and LC3 （P 〈 0. 05 ）. Conclusion：The expression of Belin-I and LC3 in hippo- campus of rats with depression induced by chronic restraint stress indicated that autophagy may be part of the mechanism of depres- sion. The effect of Xiaoyaosan Decoction on depression could be related to the regulation of Belin-1 and LC3.

关 键 词：逍遥散 抑郁症 自噬 BECLIN-1 LCA 

分 类 号：R228[医药卫生—中医基础理论]