枳椇不同部位总黄酮含量比较及其总黄酮提取工艺研究  

Comparison of the Total Flavonoids Content from Different Parts of Hovenia acerba and Extration Technology Study

作　　者：杨航[1] 裴颖[1] 孙莉佳[1] 钟宝[1] 兰光[1] YANG Hang PEI Ying SUN Li-jia ZHONG Bao LAN Guang(Jilin Agriculture Science and Technology College, Jilin 132101, China)

机构地区：[1]吉林农业科技学院,吉林吉林132101

出　　处：《保鲜与加工》2017年第4期72-76,共5页Storage and Process

基　　金：吉林省大学生科技创新项目

摘　　要：比较了枳椇各部位(叶子、枝干、果梗、果渣、种子、种壳)总黄酮的含量,获得了枳椇叶中总黄酮的最佳提取工艺。分别采用盐酸-镁粉法、三氯化铝法、醋酸铅法对枳椇各部位总黄酮含量进行定性分析,以芦丁为对照品,经Al Cl3显色和分光光度法进行定量分析,并通过正交法确定枳椇叶总黄酮的最佳提取工艺。结果表明,枳椇各部位总黄酮的含量分别为:种子5.081 mg/g、枝干3.452 mg/g、种壳0.899 mg/g、果梗0.871 mg/g、果渣0.835 mg/g,其中枳椇叶中总黄酮含量最高,为6.314 mg/g。经过正交试验分析,索氏提取法提取枳椇叶中总黄酮的最佳工艺参数为:乙醇浓度75%,料液比1∶50(g/m L),提取时间9 h,在该工艺条件下枳椇叶中总黄酮含量为14.8 mg/g。Total flavonoids concentration in different parts（including the leaves, branches, peduncles, pomace, seed,shell of seed） were compared and the extraction technology of total flavonoids from Hovenia acerba leaves was optimized by orthogonal test. HCl-Mg aluminium chloride and lead acetate methods were used to qualitatively analyze total flavonoids in different parts of Hovenia acerba, at the same time, the quantitive analysis was carried out by AlCl3 colorimetric method and spectrophotometry with rutin as reference standards. The results showted that, total flavonoids concentration in different parts of Hovenia acerba were as follows： seeds 5.081 mg/g, branches 3.452 mg/g,seed shell 0.899 mg/g, peduncles 0.871 mg/g, pomace 0.835 mg/g, and leaves 6.314 mg/g（the highest）. The best extraction process parameters were obtained by Soxhlet extraction method： ethanol content 75%, solid to liquid ratio1∶50（g/m L）, extracting time 9 h, and the total flavonoids from Hovenia acerba leaves reached 14.8 mg/g.

关 键 词：枳椇 不同部位 总黄酮 提取工艺 

分 类 号：TS201.2[轻工技术与工程—食品科学] TQ461[轻工技术与工程—食品科学与工程]