益心通络胶囊体外抗凝血的生物活性测定法研究  

Bioassay Method of Anticoagulant Research of Yixintongluo Capsule in Vitro

作　　者：肖珠[1] 王志斌[1] 程源[1] 王立娜[1] 郭玉东[2] 胡宇驰[2] 曹春然[2] 

机构地区：[1]北京中医药大学中药药理系,北京100102 [2]北京市药品检验所,北京100035

出　　处：《实验动物科学》2017年第3期20-23,共4页Laboratory Animal Science

摘　　要：目的考察以凝血酶时间(TT)为反映体系建立益心通络胶囊体外抗凝血的生物活性测定法的可行性。方法将不同浓度的益心通络胶囊浸提液加入兔血浆中,测定凝血酶时间(TT),观察益心通络胶囊浸提液浓度与TT值的量效关系。规定浓度为0.261 g/m L的益心通络胶囊溶液使空白血浆(加0.9%生理盐水)凝血酶时间延长1个百分点定义为1个效价单位(U),定义并测得益心通络胶囊标准品效价。结果当益心通络胶囊浸提液在0.1886~0.2610 g/m L浓度范围内时,凝血酶时间与浓度线性较好(r=0.9980;0.9985;0.9829);测得益心通络胶囊标准品的效价为2395 U/g。结论益心通络胶囊体外凝血酶时间的生物活性测定方法具有可行性。Objective To inspection the feasibility of bioassay method of anticoagulant research of YiXinTongLuo capsule in vitro. Method Different concentrations of aqueous extract of YiXINTongLuo capsule were mixed with Rabbit plasma and the TT was determined by coagulometer. One titer unit（U） was defined as one second longer for anticoagulant research of YiXinTongLuo capsule than the coagulation time of the blank control, and the titer of YiXinTongLuo capsule standard substance was defined and measured. Result Within the range of 0. 1886 0. 2610 g/mL, TT and the aqueous extract concentration showed a good linear relationship respectively（ r = 0. 9980; 0. 9985 ;0. 9829）. The titer of the standard substance of YiXinTongLuo capsule was measured to be 2395 U/g. Conclusion It is feasibility that TT used toestablish bioactivity assay in vitro.

关 键 词：生物活性测定法 凝血酶时间 益心通络胶囊 

分 类 号：R965.2[医药卫生—药理学]