G0S2基因在胶质瘤中的表达及生物学意义  

Expression of G0S2 gene in glioma and its biological significance

作　　者：谢琳[1] 李凯舒 刘安民[1] 

机构地区：[1]中山大学孙逸仙纪念医院神经外科,广州510120 [2]广州医科大学附属第六医院神经外科,广东清远511515

出　　处：《岭南现代临床外科》2017年第4期381-386,共6页Lingnan Modern Clinics in Surgery

基　　金：广东省科技发展专项基金(2016A020215061)

摘　　要：目的分析G0S2(G0S2G0/G1 switch 2)基因与胶质瘤预后及恶性程度的相关性。方法应用生物信息学技术对GSE33331芯片进行分析得出差异性表达的基因G0S2,并使用DAVID、TCGA数据库(The Cancer Genome Atlas)对该基因进行GO(Gene Ontology)分析及生存分析,使用实时荧光定量PCR方法研究G0S2与胶质瘤级别的相关性。结果生物信息学分析结果提示G0S2是芯片中表达差异第3显著的基因(log FC=-2.53672,P<0.001),该基因参与肿瘤的脂质、分子等代谢过程,高表达该基因的胶质瘤患者生存时间显著下降(Chisq=150,P<0.001)。此外通过40例胶质瘤的q PCR结果证实G0S2在高级别与低级别胶质瘤中差异表达(t=3.168,P=0.003)。结论 G0S2基因表达水平与胶质瘤恶性程度及预后相关。Objective To analyze the correlation between G0S2（G0S2G0/G1 switch 2）gene and glioma prognosis and malignancy. Methods The GSE33331 chip was analyzed by bioinformatics technique and the differential expression gene G0S2 was obtained. GO（Gene Ontology）analysis and survival analysis of G0S2 gene were performed using DAVID and TCGA database（The Cancer Genome Atlas）. The correlation between G0S2 and glioma level was studied by real-time fluorescence quantitative PCR. Results Bioinformatics analysis showed that G0S2 was the third most significant gene in the chip（log FC=-2.53672,P〈0.001）. The gene was involved in the metabolism of lipids and molecules,and the survival time of glioma patients with high expression of this gene was significantly decreased（Chisq=150,P〈0.001）. In addition,40 cases of glioma with q PCR results confirmed that G0S2 was differentially expressed in high and low grade gliomas（t=3.168,P=0.003）. Conclusion The expression level of G0S2 gene is related to the degree of malignancy and prognosis of glioma.

关 键 词：G0S2基因 胶质瘤 恶性程度 预后 

分 类 号：R739.41[医药卫生—肿瘤]