扶脾化瘤饮对人胃癌裸鼠移植瘤生长及TNF、NK活性的影响  

Effect of Fupi Hualiu decoction on subcutaneously implanted human gastric cancer in nude mice

作　　者：杨洁[1] 杨承祖[1] 宋延平[1] 姜姗姗[1] 

机构地区：[1]陕西省中医药研究院,西安710003

出　　处：《陕西中医》2017年第8期1142-1143,1150,共3页Shaanxi Journal of Traditional Chinese Medicine

基　　金：陕西省科技攻关计划项目(2010K14-03-12)

摘　　要：目的:观察中药复方扶脾化瘤饮对荷人胃癌SGC-7901裸鼠移植瘤生长及对荷瘤裸鼠TNF、NK活性的影响。方法:建立人胃癌SGC-7901细胞BALB/C裸小鼠皮下移植瘤模型,随机分为5组,每组8只。荷瘤对照组予纯净水灌胃,扶脾化瘤饮大、中、小剂量组予扶脾化瘤饮煎剂灌胃,20ml/kg,1次/d;阳性对照组予环磷酰胺灌胃,20mg/kg,1次/d。观察各组裸鼠移植瘤生长情况,采用L929细胞杀伤实验检测TNF活性及NK细胞活性。结果:扶脾化瘤饮大、中剂量对荷人胃癌SGC-7901裸鼠具有显著的抑瘤作用,抑瘤率为44.46%、35.79%(P<0.01,P<0.05),同时能显著促进产生TNF,细胞毒指数分别为55.53%、52.52%(P<0.01);扶脾化瘤饮大剂量对脾脏NK细胞活性也有提高作用,细胞杀伤率为30.59%(P<0.05)。结论:扶脾化瘤饮可以抑制人胃癌SGC-7901的生长,且能拮抗荷瘤所致的免疫功能的抑制。Objective： To investigate the effects of Fupi Hualiu decoction,a Chinese herbal formula,on tumor growth and the activity of TNF and NK. Methods： Forty BALB/C nude mice transplanted with SGC-7901 cells were randomly divided into five groups（8 mice per group）,and intragastric administered with normal saline or Fupi Hualiu decoction（20ml/kg）,or cyclophosphamide（20mg/kg） daily.Tumor volume were observed.Activity of TNF、NK were detected by L929 cell killing test. Results： Fupi Hualiu decoction of high and media groups had obvious inhibition to the tumor volume compared with controls, the tumor inhibition rate was 44.46%（P〈0.01） and 35.79%（P〈0.05）.The production of TNF in mice bearing tumor was also enhanced significantly, Fupi Hualiu at the dose as above had cytotoxicity index of 55.53%、52.52%（P〈0.01）.The activity of NK cell could be increased by Fupi Hualiu at high dose, the killing ratio was 30.59%（P〈0.05）.Conclusion： Fupi Hualiu decoction has inhibitory effects on tumor growth in SGC-7901 gastric carcinoma,and has antagonism action to the immunosuppressive caused by tumor.

关 键 词：胃肿瘤/中医药疗法 @扶脾化瘤饮 肿瘤坏死因子 小鼠 裸 

分 类 号：R735.2[医药卫生—肿瘤]