人胎盘部位滋养细胞肿瘤细胞系的建立及其鉴定  

Establishment and identification of human placental site trophoblastic tumor cell line

作　　者：刘齐雨 李可[1,2] 胥婧[1,2] 黄云柯 赵晨燕[3] 罗建民[4] 李桂玲[1,2] 康玉[1,2] 徐丛剑[1,2] 

机构地区：[1]复旦大学附属妇产科医院中西医结合科,上海200011 [2]上海市女性生殖内分泌相关疾病重点实验室,上海200011 [3]复旦大学附属妇产科医院病理科,上海200011 [4]复旦大学生物医学影像研究中心,上海200032

出　　处：《中国癌症杂志》2017年第7期521-526,共6页China Oncology

基　　金：国家自然科学基金(81472423);上海市科委国际合作项目(14430723000)

摘　　要：背景与目的:胎盘部位滋养细胞肿瘤(placental site trophoblastic tumor,PSTT)是一种特殊类型的妊娠滋养细胞肿瘤。该研究旨在建立人PSTT细胞系,鉴定其基本特性。方法:通过手术切除的PSTT组织标本原代培养,纯化并传代,建立人PSTT细胞系。鉴定方法包括:观察细胞形态,CCK-8法绘制细胞生长曲线,进行染色体核型分析,细胞与肿瘤组织短串重复序列(short tandem repeat,STR)分析,以及免疫组织化学染色。结果:建立稳定传代的人PSTT细胞系PSTT-1。PSTT-1细胞单层贴壁生长,失去接触抑制性,呈多突起的纺锤形结构。体外培养生长良好,每5 d传代1次,目前已传至30余代。染色体核型分析结果为正常女性核型:46,XX。PSTT-1与肿瘤组织STR分析结果高度匹配。PSTT-1细胞表达β-catenin、CD146、人绒毛膜促性腺激素(human chorionic gonadotrophin,hCG)、人胎盘生乳素(human placental lactogen,hPL)和Muc4。结论:该研究成功建立人PSTT细胞系PSTT-1,为PSTT的研究提供了体外实验模型。Background and purpose： Placental site trophoblastic tumor（PSTT） is a special type of gestational trophoblastic tumor. It remains no general cell line for PSTT. The aim of this study was to establish and identify a human PSTT cell line. Methods： In this study, a PSTT cell line（PSTT-1） was derived from primary culture of a surgically resected PSTT tissue. We further identified the basic characteristics of PSTT-1, including cell morphology, cell growth curve, karyotype analysis, short tandem repeat（STR） profiling of PSTT-1 and its parental tumor, and immunohistochemistry analysis. Results： A human PSTT cell line named PSTT-1 was successfully established. PSTT-1 formed a monolayer growth and lost contact inhibition, and exhibited a pleomorphic morphology. PSTT-1 cells grew actively in vitro. PSTT-1 was passaged every 5 days, and maintained in culture for more than 30 passages. Karyotyping of PSTT-1 revealed normal female karyotype： 46, XX. STR profiling of PSTT-1 was nearly identical to itsparental tumor. Immunohistochemistry analysis of PSTT-1 revealed expression of β-catenin, CD146, human chorionic gonadotropin（hCG）, human placental lactogen（hPL） and Muc4. Conclusion： This study established a PSTT cell line, which could be useful for further research on PSTT.

关 键 词：PSTT 细胞系 原代培养 

分 类 号：R737.33[医药卫生—肿瘤]