Lgr5及SOX-9在宫颈癌组织中的表达及其与预后的关系  

The expressions and clinical significance of Lgr5 and SOX-9 in cervical carcinoma

作　　者：潘静[1] 许艳茹[1] 沈娟娟[1] 

机构地区：[1]新疆医科大学第二附属医院妇产科,新疆乌鲁木齐830028

出　　处：《临床和实验医学杂志》2017年第15期1492-1495,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨含亮氨酸重复序列G-蛋白偶联受体5(Lgr5)及性别决定区Y框蛋白9(SOX-9)在宫颈癌中的表达与临床意义。方法选取手术治疗的宫颈癌患者140例,同时选取同期因宫颈良性病变行肿物剥除或附件切除的40例患者作为对照组。应用免疫组化(S-P)方法检测140例宫颈癌组织(宫颈癌组)、140例宫颈癌癌旁组织(癌旁组)、正常宫颈组织40例(正常组)中的Lgr5及SOX-9蛋白表达,分析其与宫颈癌患者临床病理指标的关系。结果Lgr5及SOX-9在正常宫颈组织、癌旁组织和宫颈癌中的阳性率比较,差异具有统计学意义(P<0.05);Lgr5表达与肿瘤分化程度、淋巴结转移及TNM分期有关(P<0.05),SOX-9表达与肿瘤分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05)。Lgr5与SOX-9呈正相关(r=15.27,P<0.05)。SOX-9蛋白和Lgr5蛋白(+++)表达者的5年生存率及中位生存时间均较(+^++)表达者低或短,差异具有统计学意义(P<0.05)。结论 Lgr5及SOX-9的增强表达与宫颈癌侵袭性增强有密切关系,其表达可作为判断宫颈癌患者预后的指标。Objective To study Lgr5 and SOX - 9 expression in cervical carcinoma, and analyze the clinical significance. Methods Im- munohistochemistry staining method was performed in 140 patients with cervical carcinoma and 40 patients with benign cervical tumor in our hospi-tal. Lgr5 and SOX - 9 in the cervical carcinoma tissues （ cervical carcinoma group ,140 cases） , para - carcinoma tissues （ para - carcinoma group, 140 cases） and normal cervical tissues （normal group, 40 cases） were detected with immunohistochemical method. The correlation with clinic opathological parameters of cervical carcinoma were also analyzed. The study protocol was approved by the Ethical Review Board of Investigation. Informed consent was obtained from each participant. Results There was significant difference among control group, surrounding tissue and cervi-cal carcinoma of the positive expression rate of Lgr5 and SOX - 9 （ P 〈 0 .0 5 ） . The expression of Lgr5 was related with stage, the degree of tumor differentiation , lymph node metastasis and TNM （ P 〈 0. 0 5 ） . The expression of SOX - 9 was related with stage and the degree of tumor differenti-ation ,depth of invasion, lymph node metastasis and TNM （ P 〈 0 .0 5 ） . There was a positive correlation between Lgr5 and SOX - 9 （ r = 1 5.2 7 , P 〈 0. 05）. There was a significant difference of the five - year overall survival （ OS） rates for patients with high Lgr5 and SOX - 9 expression （ ＋ ＋ ＋） and low expression （＋）？（ ＋ ＋）（ P 〈0. 05）. Conclusion The up - regulated expression level of Lgr5 and SOX - 9 was closely related to the increased malignancy and invasion of tumors. The expression of protein may act as an indicator for the prognosis of patients with cervical car-cinoma.

关 键 词：宫颈癌 LGR5 SOX-9 肿瘤浸润 

分 类 号：R737.33[医药卫生—肿瘤]