不同分期原发性肝癌患者肿瘤组织MVD与Survivin表达的相关性研究  

Study on the relationship between MVD and Survivin expression in different stages of primary hepatocellular carcinoma

作　　者：党存曙[1] 张帆[1] 杨明[2] 边佺[3] 王涵[1] 

机构地区：[1]中国石油天然气集团公司中心医院肝胆外科,河北廊坊065000 [2]廊坊市人民医院,河北廊坊065000 [3]天津医院,天津301900

出　　处：《临床和实验医学杂志》2017年第16期1584-1588,共5页Journal of Clinical and Experimental Medicine

基　　金：廊坊市科技支撑计划项目(2011013036)

摘　　要：目的探讨不同分期原发性肝癌患者肿瘤组织微血管密度(MVD)与Survivin表达的相关性。方法选取接受原发性肝癌手术治疗的患者40例,采用免疫组织化学法检测不同分期肿瘤组织、癌旁组织以及正常肝脏组织中Survivin和CD34单克隆抗体标记的MVD的表达。结果肿瘤组织中Survivin的阳性表达率高于癌旁组织中Survivin的阳性表达率(P<0.05);肿瘤组织Survivin阳性表达率随TNM分期升高而增加(P<0.05);癌旁组织Survivin阳性表达率随TNM分期升高而增加(P<0.05);肿瘤组织MVD表达水平高于癌旁组织(P<0.05);TNM分期为Ⅰ、Ⅱ期肿瘤组织中MVD表达水平低于Ⅲ、Ⅳ期肿瘤组织(P<0.05);TNM分期为Ⅰ、Ⅱ期癌旁组织中MVD表达水平低于Ⅲ、Ⅳ期癌旁组织(P<0.05);肿瘤组织中Survivin表达阳性组MVD水平高于Survivin表达阴性组MVD水平(P<0.05);肝癌肿瘤组织中Survivin表达与MVD呈正相关关系(P<0.05)。结论原发性肝癌患者肿瘤组织MVD与Survivin表达水平与临床TNM分期有关,TNM分期越高,MVD与Survivin表达水平越高,且Survivin表达水平与MVD表达水平正相关,Survivin可能参与了肝癌的血管形成过程。Objective To investigate the relationship between microvessel density（ MVD） and Survivin expression in different stages of primary hepatocellular carcinoma. Methods 40 patients with primary hepatocellular carcinoma treated with surgery in our hospital were selected.Immunohistochemistry was used to detect the expression of Survivin and CD34 monoclonal antibody labeled MVD in the different stages of tumor tissues,paracancerous tissue and normal liver tissues. Results The positive expression rate of Survivin in tumor tissues was higher than that in the paracancerous tissue（ P 〈 0. 05）. The positive expression rate of Survivin in tumor tissues increased with the increase of TNM stage（ P 〈 0. 05）.The positive expression rate of Survivin in paracancerous tissue increased with the increase of TNM stage（ P 〈 0. 05）. The expression level of MVD in tumor tissues was higher than that in paracancerous tissue（ P 〈 0. 05）; the expression level of MVD in TNM stage I and II tumor tissues was lower than that of stage III and IV tumors（ P 〈 0. 05）. The expression level of MVD in TNM stage I and II paracancerous tissue was lower than that of stage III and IV tumors（ P 〈 0. 05）. The MVD expression level in tumor tissues of Survivin positive group was significantly higher than that in Survivin negative group（ P 〈 0. 05）. The expression of Survivin in hepatocellular carcinoma tissues was positively correlated with MVD（ P 〈 0. 05）. Conclusion The expression of MVD and Survivin in tumor tissues of patients with primary hepatocellular carcinoma was related to the clinical stage of TNM. The higher the TNM stage,the higher the expression level of MVD and Survivin,and the expression level of Survivin was positively correlated with MVD expression. Survivin may be involved in the process of angiogenesis in primary hepatocellular carcinoma.

关 键 词：原发性肝癌 分期 MVD SURVIVING 相关性 

分 类 号：R735.7[医药卫生—肿瘤]