桑黄多糖对肉瘤S180细胞体内外的抑瘤作用  

Antitumor Effect of Phellinus Linteus Polysaccharide on Sarcoma S180 Cells in vivo and in vitro

作　　者：刘燕琳[1] 刘海燕[2] 常金[2] 党和勤[1] 

机构地区：[1]泰山医学院附属医院药剂科,山东泰安271000 [2]泰山医学院附属医院肿瘤科,山东泰安271000

出　　处：《中国药房》2017年第22期3069-3072,共4页China Pharmacy

基　　金：山东省中医药科技发展计划项目(No.2015-264)

摘　　要：目的:研究桑黄多糖对肉瘤S180细胞体内外的抑瘤作用。方法:选用对数生长期肉瘤S180细胞,加入0(空白对照)、2、4、8 mg/m L的桑黄多糖溶液,分别培养12、24、36、48 h,MTT法测定细胞体外增殖抑制率,荧光染色法观察细胞凋亡形态,流式细胞术检测细胞凋亡率。建立S180细胞荷瘤小鼠模型并随机分为对照组和桑黄多糖高、中、低剂量组(400、200、100 mg/kg),每组10只,每天ig相应药物1次,连续12 d;末次给药24 h后处死小鼠,称瘤质量,计算抑瘤率,免疫组化法检测肿瘤组织中抑癌基因PTEN与癌基因C-myc蛋白表达。结果:与空白对照比较,桑黄多糖能增高S180细胞的增殖抑制率,促进其凋亡率升高(P<0.05或P<0.01),且具有浓度与时间依赖性。与对照组比较,桑黄多糖各剂量组小鼠的抑瘤率明显升高(P<0.01),PTEN蛋白表达增强(P<0.05或P<0.01),C-myc蛋白表达减弱(P<0.05)。结论:桑黄多糖具有体内外抑瘤作用,并能上调抑癌基因PTEN和下调癌基因C-myc的蛋白表达。OBJECTIVE：To study the antitumor effect of phellinus linteus polysaccharide on sarcoma S180 cells in vivo and in vitro. METHODS：Sarcoma S180 cells in logarithmic growth period were selected,adding into 0（blank control）,2,4,8 mg/m L phellinus linteus polysaccharide solution and respectively culturing for 12,24,36,48 h. The in vitro proliferation inhibition rate of cells was determined by MTT method;its apoptotic morphology was observed by fluorescence staining and cell apoptosis rate was detected by flow cytometry. S180 tumor-bearing mice models were established and randomly divided into control group,phellinus linteus polysaccharide high-dose,medium-dose,low-dose groups（400,200,100 mg/kg）,10 in each group. Model mice were intragastrically administrated related medicined,once a day,for 12 d. Mice were executed after 24 h of last administration,tumor weight was determined,tumor inhibition rate was calculated. Immunohistochemistry was conducted to detect the tumor suppressor gene PTEN and oncogene C-myc protein expressions in tumor tissue. RESULTS：Compared with blank control group,phellinus linteus polysaccharide can increase the proliferation inhibition rate of S180 cells and induce the increase of apoptosis rate（P〈0.05 or P〈0.01）,showing a concentration-time manner. Compared with control group,the tumor inhibition rates in phellinus linteus polysaccharide groups were obviously increased（P〈0.01）,PTEN protein expressions were strengthened（P〈0.05 or P〈0.01）and C-myc protein expressions were weakened（P〈0.05）. CONCLUSIONS：Phellinus linteus polysaccharide shows antitumor effect in vivo and in vitro,which can up-regulate the PTEN,down-regulate C-myc protein expressions.

关 键 词：桑黄多糖 抑瘤作用 体内 体外 肉瘤S180细胞 小鼠 

分 类 号：R361.3[医药卫生—病理学]