盐度对一株淡水栅藻Scenedesmus sp.生长及生化组成的影响  

Effects of Salinity on the Growth and Biochemical Properties of a Freshwater Algae Scenedesmus sp.

作　　者：李嘉颖[1,2] 李涛[1] 谭丽[1,2] 吴嘉仪[1] 向文洲[1] 刘德海 LI Jia-ying LI Tao TAN Li WU Jia-yi XIANG Wen-zhou LIU De-hai(Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy ofScienees, C, nangzhou 510301 University of Chinese Academy of Scienees, Beijing, 100049 Guangzhou Baiyun Lianjia Fine Chemical Factory, Guangzhou 510412)

机构地区：[1]中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室,广州510301 [2]中国科学院大学,北京100049 [3]广州市白云联佳精细化工厂,广州510412

出　　处：《生物技术通报》2017年第7期155-161,共7页Biotechnology Bulletin

基　　金：广东省海洋渔业科技攻关与研发项目(A201601A13);中国科学院可再生能源重点实验室基金(Y607k91001;Y507jb1001);广东丹姿集团科技研发项目(DZ201501)

摘　　要：通过管式光生物反应器培养,分析比较不同盐度(0-40‰)对一株淡水栅藻(Scenedesmus sp.)生长和生化特性影响,评价海水培养该藻株的产业化潜力,探讨其高盐适应机制,为进一步海水驯化研究提供理论参考。采用显微观察、索氏提取法、凯氏定氮法、苯酚硫酸法、气相色谱法及高效液相色谱法分别测定微藻生长及蛋白质、油脂、多糖含量以及色素、脂肪酸组成情况。结果表明,高盐条件下藻细胞明显增大,并出现自然沉降现象,可实现低成本采收。随盐度增加,栅藻生长逐步被抑制,40‰盐度条件下生长完全停止,但在3‰盐度下仍良好生长,培养末期生物量可达2.84 g/L。30‰盐度组蛋白质含量相对淡水组提高95.40%、产率与淡水组相近,加之高盐培养可较大幅度降低蛋白质的生产成本,因此,采用海水培养栅藻来开发蛋白资源具有一定的潜力。研究还表明,30‰盐度下藻细胞内β-胡萝卜素、虾青素、可溶性多糖等成分大量积累,可能是其适应高盐胁迫的重要生理基础。The aims of this work are to analyze and compare the effects of different salt concentration（0-40‰）on the growth andbiochemical characteristics of a freshwater algae Scenedesmus sp. incubated in small tubular bioreactors,and evaluate the industrializationpotential of culturing this algae in seawater as well explore the adaptive mechanism at high salinity,which provides theoretical references forfurther study on seawater domestication. The growth of the microalgae,the content of protein,lipid and polysaccharide,and the compositionof pigments and fatty acids were analyzed correspondingly by microscopic observation,Soxhlet extraction,Kjeldahl method,phenol sulfuricacid determination,gas chromatography technology,and HPLC. The results showed that cells grew bigger and naturally precipitated at highsalinity,meaning that it can be harvested at low cost. The study also found that growth of Scenedesmus sp. was gradually inhibited with theincrease of salinity,and completely inhibited at the salinity of 40‰;however,it still grew well at the salinity of 30‰ with biomass of 2.84 g/L,and higher protein content（95.40% higher than 0‰ group）with similar protein productivity as that by freshwater. Combined with the greatcost reduction of producing protein at high salinity,culturing Scenedesmus sp. in seawater for protein resource is of high potential. Moreover,with the increase of salinity,Scenedesmus sp. tended to accumulate more β-carotene,astaxanthin,and even soluble sugar,which might bethe key physiological basis of adapting salt-stress.

关 键 词：淡水栅藻 SCENEDESMUS sp. 高盐胁迫 生长 生化组成 

分 类 号：Q945[生物学—植物学]