氨基酸脱氢酶的催化机理、分子改造及合成应用  

Catalysis,engineering and application of amino acid dehydrogenases

作　　者：陈曦[1] 高秀珍[2] 朱敦明[1] Xi Chen Xiuzhen Gao Dunming Zhu(National Engineering Laboratory for Industrial Enzymes, Tianjin Engineering Research Center of Biocatalytic Technology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China College of Life Sciences, Shandong University of Technology, Zibo 255000, Shandong Province, China)

机构地区：[1]中国科学院天津工业生物技术研究所,工业酶国家工程实验室,天津市生物催化技术工程中心,天津300308 [2]山东理工大学生命科学学院,山东淄博255000

出　　处：《微生物学报》2017年第8期1249-1261,共13页Acta Microbiologica Sinica

基　　金：国家自然科学基金(21472232,21402109)~~

摘　　要：氨基酸脱氢酶催化可逆的氨基酸氧化脱氨和酮酸的不对称还原胺化反应,热力学上反应平衡倾向于生成氨基酸方向,从原子经济学和对环境影响的角度来看,是具有极大优势的氨基酸合成方法之一。本文将主要阐述近年来在?-氨基酸脱氢酶催化机理、分子改造和合成应用方面的研究进展。Amino acid dehydrogenases catalyze the reversible oxidative deamination of amino acids and reductive amination of keto acids, with thermodynamic equilibrium favoring the reductive amination direction. This is one of the most attractive methods for the synthesis of amino acids with high atomic economy and less environmental impact. This review addresses recent advances in studies on reaction mechanism, molecular modification and synthetic applications of amino acid dehydrogenases.

关 键 词：L-氨基酸脱氢酶 D-氨基酸脱氢酶 催化机理 酶工程 合成应用 

分 类 号：Q55[生物学—生物化学]