正交法优选亮菌多糖脱色工艺研究  

Study on Decoloration Technique of Armillariella Polysaccharide by Orthogonal Experiments

作　　者：黄纯[1] 王小平[1] 程海涛[1] 姜晟[1] HUANG Chun WANG Xiao-ping CHENG Hai-tao et al(China Pharmaceutical University,Nanjing,Jiangsu 211196)

机构地区：[1]中国药科大学,江苏南京211196

出　　处：《安徽农业科学》2017年第21期133-134,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立亮菌多糖脱色的最佳方法。[方法]以色素脱除率作为主要考察指标,通过单因素考察和正交法优化亮菌多糖脱色工艺。[结果]D152树脂、45℃、p H 5.0为最佳脱色条件。[结论]D152作用后色素脱除率高,糖保留率高,有益于下一步分离纯化。[ Objective ] The research aimed to establish the optimum method of decoloration about ArmillarieUa polysaccharide. [ Method ] Taking the decoloring ratio as main index, the optimum decoloring technique were established through single factorial test and orthogonal experiments. [ Result ] The best method of decoloration were obtained as follows： I）152 resins working in pH 5.0 and 45 ℃. [ Conclusion] The decoloring ratio of D152 and the reteion rate of polysaccharide are high, which is beneficial to the next step.

关 键 词：脱色素 亮菌多糖 工艺优化 正交法 

分 类 号：S567.3[农业科学—中草药栽培]