高脂饮食影响小鼠体内能量代谢的性别差异  

Difference of energy metabolism between male and female mice exposed to high fat diet

作　　者：崔菊[1] 陈爱群[1] 庞婧[1] 李瑾[1] 李云炫 张铁梅[1] 蔡剑平[1] CUI Ju CHEN Aiqun PANG Jing LI Jin LI Yunxuan ZHANG Tiemei CAI Jianping(MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, China)

机构地区：[1]北京医院国家老年医学中心卫生部北京老年医学研究所卫生部老年医学重点实验室,北京100730

出　　处：《山西医科大学学报》2017年第7期658-664,共7页Journal of Shanxi Medical University

基　　金：国家自然科学基金青年项目(31400995);北京医院"科技新星"项目(BJ-2016-034)

摘　　要：目的比较高脂喂饲小鼠影响体内能量代谢的性别差异,探讨其可能机制。方法 C57BL/6小鼠分为4组,雌、雄鼠各两组,每组各10只。其中一组雄鼠和一组雌鼠喂饲正常饲料,另外一组雄鼠和一组雌鼠喂饲60%高脂饲料12周。观察各组小鼠的体质量、血清生化水平、24 h内能量消耗的改变;采用Western blot检测小鼠棕色脂肪组织中解偶联蛋白1(UCP1)和马达蛋白KIF5B的表达水平。结果与雌鼠比较,高脂喂饲条件下,雄鼠的体质量增长(P<0.05);葡萄糖耐受水平降低(P<0.05);空腹血糖、血清胰岛素、瘦素和肿瘤坏死因子-α水平均显著上升(P<0.05);血清甘油三酯、总胆固醇和脂联素水平无统计学差异(P>0.05);能量消耗显著下降(P<0.05),呼吸熵和活动能力没有统计学差异(P>0.05);棕色脂肪组织中UCP1和KIF5B的表达下降。结论雄性小鼠在高热量饮食下更容易肥胖,雌鼠棕色脂肪组织通过较强的产热功能,消耗多余能量,维持机体能量代谢平衡。Objective To compare the difference of energy metabolism between male and female mice,and to explore the possible mechanism of gender difference in metabolic disorders. Methods The C57BL/6 mice were divided into 4 groups（ n = 10 each） ： two groups of male mice and two groups of female mice. The mice in one male group and one female group were fed with standard chow diet,and the mice in the other groups were fed with 60% high fat diet for 12 weeks. The body weight,serum biochemical levels and 24 h energy consumption in the mice were observed. Western blot was used to detect the expression of KIF5 B and UCP1 in brown adipose tissues. Results After fed with high fat diet,the body weight gain was significantly higher in the male mice than that of female mice（ P 〈0. 05）,and the levels of fating blood glucose,serum insulin,leptin,and TNF-α were also significantly higher（ P〈 0. 05）,while the glucose tolerance level was lower（ P 〈0. 05）. There was no statistical difference in the levels of serum triglyceride,total cholesterol and adiponectin between male and female mice after fed with high fat diet（ P 〉0. 05）. The energy expenditure was significantly lower in the male mice compared with the female mice after fed with high fat diet（ P 〈0. 05）. There was no statistical difference in the respiratory quotient and ambulatory activities between the male mice and female mice after fed with high fat diet（ P 〈0. 05）. Western blot results revealed that the expression levels of KIF5 B and UCP1 in brown adipose were higher in male mice than that in female mice after fed with high fat diet. Conclusion Compared with female mice,male mice have a higher susceptibility to obese on the condition of high fat diet. The brown adipose of the female mice have stronger thermogenice function which can help the body to consume excess energy and maintain energy metabolic balance.

关 键 词：高脂饮食 能量代谢 性别差异 马达蛋白 

分 类 号：R589.2[医药卫生—内分泌]