microRNA-211在脑胶质瘤中的表达及其与临床病理预后的关系  

The expression of microRNA-211 in glioma tissues and its value in predicting prognosis

作　　者：梅喜平[1,2] 郑鲲[1,2] 邵碧波[1,2] 袁利学[1,2] 冯辉斌[1,2] MEI Xiping ZHENG Kun SHAO Bibo YUAN Lixue FENG Huibin(Department of Intensive Care Unit, Huangshi Cen- tral Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi 435000, China Hubei Key Labo- ratory of Kidney Disease Pathogenesis and Intervention, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group)

机构地区：[1]鄂东医疗集团黄石市中心医院,湖北理工学院附属医院重症医学科,黄石435000 [2]鄂东医疗集团黄石市中心医院,湖北理工学院附属医院肾脏疾病发生与干预湖北省重点实验室

出　　处：《山西医科大学学报》2017年第7期711-714,共4页Journal of Shanxi Medical University

摘　　要：目的探讨胶质瘤组织中microRNA-211(miR-211)的表达及其在预测胶质瘤患者预后中的价值。方法 qRT-PCR法检测52例神经胶质瘤组织以及12例正常脑组织中miR-211的表达水平,分析其与胶质瘤患者临床病理特征及其预后之间的关系。结果胶质瘤组织中miR-211的表达水平(0.41±0.24)明显低于正常脑组织(1.00±0.13,P<0.001),并且与WHO分级呈负相关趋势(WHOⅢ-Ⅳ,0.21±0.14 vs WHOⅠ-Ⅱ,0.60±0.18,P=0.016)。胶质瘤组织中miR-211的表达水平与WHO分级和KPS评分密切相关,而与患者年龄、性别、肿瘤位置、肿瘤直径等均无关。miR-211高表达组患者的生存率明显高于miR-211低表达组,差异有统计学意义(P=0.037)。胶质瘤的WHO分级增高、KPS评分<80分及miR-211低表达均是影响胶质瘤患者预后的危险因素。结论 miR-211在胶质瘤组织中呈低表达,与胶质瘤患者预后显著相关,可作为预测胶质瘤患者预后的独立因子及治疗新靶点。Objective To investigate the expression of microRNA-211（ miR-211） in glioma tissues and assess its relationship with prognosis of glioma patients. Methods RT-PCR was performed to examine the expression level of miR-211 in 52 glioma tissues and 12 normal brain tissues. The correlation of miR-211 with clinicopathological factors and prognosis was investigated. Results The expression of miR-211 was significantly lower in glioma tissues than that in normal brain tissues（ 0. 41 ± 0. 24 vs 1. 00 ± 0. 13,P〈 0. 001）,and negatively associated with advanced WHO classification（ WHO Ⅲ-Ⅳ0. 21 ± 0. 14 vs WHO Ⅰ-Ⅱ0. 60 ± 0. 18,P =0. 016）. The miR-211 expression level was significantly lower in patients with the advanced WHO pathological grade of glioma and low KPS. However,the miR-211 expression level was not significantly associated with age,gender,tumor location,or tumor diameter.Furthermore,the overall survival rate of glioma patients with low miR-211 expression was significantly lower than that of patients with high miR-211 expression（ P = 0. 037）. Advanced WHO grade,KPS score less than 80 scores,and low expression of miR-211 were the independent risk factors for predicting the poor prognosis of glioma patients. Conclusion The miR-211 is down-regulated in glioma tissues and associated with the prognosis of glioma patients. The miR-211 might be an independent prognostic indicator and a potential therapeutic target for glioma patients.

关 键 词：胶质瘤 microRNA-211 预后 

分 类 号：R730.264[医药卫生—肿瘤]