蓖麻提取液对甜菜夜蛾幼虫解毒酶活性的影响  

Effect of ricinine on the activities of detoxifying enzymes in Spodoptera exigualarvae

作　　者：化丽丹[1,2] 蒋杰贤[1] 季香云[1] 万年峰[1] 张浩[1] 杨益众[3] Hua Lidan Jiang Jiexian Ji Xiangyun Wan Nianfeng Zhang Hao Yang Yizhong(Institute of Eco-Environment Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China Taizhou Entry-exit Inspection and Quarantine Bureau, Taizhou 225300, China College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China)

机构地区：[1]上海市农业科学院生态环境保护研究所,上海201403 [2]江苏省泰州出入境检验检疫局,泰州225300 [3]扬州大学园艺与植物保护学院,扬州225009

出　　处：《植物保护》2017年第4期81-84,103,共5页Plant Protection

基　　金：上海市科技兴农推广项目[沪农科推字(2016)第2-2-8号];上海市科技兴农重点攻关项目[沪农科攻字(2016)第3-4-2号]

摘　　要：为探讨植物提取液对甜菜夜蛾解毒酶系活性的影响效应,采用浸渍法测定了不同浓度蓖麻碱(3.80、1.90、0.95、0.48和0.24g/L)对甜菜夜蛾幼虫碱性磷酸酯酶(AKP)、酸性磷酸酯酶(ACP)和谷胱甘肽S-转移酶(GSTs)活性的影响。结果表明:与清水对照组相比,不同浓度处理48h和72h对AKP的抑制作用均达到显著水平,而3.80g/L处理24h即可显著抑制AKP活性;与对照组相比,不同浓度蓖麻碱处理72h均会显著降低其ACP活性,而浓度大于0.24g/L时处理24h和48h即可显著降低ACP活性;蓖麻碱处理组GSTs活性在处理后24、48和72h都显著低于对照组,但不同浓度间无显著差异;本研究揭示了蓖麻碱对这3种解毒酶均有较强的抑制作用,且抑制作用随蓖麻碱浓度升高和作用时间延长而增强。To investigate the effect of plant extracts on the detoxifying enzymes of noctuid,we used the dipping method to determine the effects of the five different concentrations of ricinine（3.80、1.90、0.95、0.48 and0.24 g/L）on alkaline phosphatase（AKP）,acid phosphatase（ACP）and glutathione S-transferase（GSTs）.The results indicated that compared to the control group（fresh water treatment）,the treatment of ricinine significantly inhibited the activities of AKP at 48 and72 h after;while the treatment of 3.80 g/L of ricinine could inhibit the activity of AKP at 24 h post treatment.The activities of ACP were also significantly inhibited in all five ricinine-treated groups at 72 h after treatment,while the treatments with concentrations higher than0.24 g/L significantly inhibited the activity of ACP at 24 and48 h after treatment.The activities of GSTs were all significantly inhibited in all treatments compared to that of the control,but no difference was found among treatments of different concentrations.These results indicated that ricinine had a strong inhibitory effect on the three enzymes tested and the inhibition were concentration-and time-dependent.

关 键 词：蓖麻碱 甜菜夜蛾 幼虫 解毒酶 抑制作用 

分 类 号：S482.39[农业科学—农药学]