超声联合微泡实验参数对不同组织肿瘤细胞生物效应的影响  

Bioeffect of different parameters on four tumor cell lines by ultrasound-mediated microbubble destruction

作　　者：时丹丹[1] 李杰[1] 段素娟[1] 郭鲁[1] 商蒙蒙 宁松[1] 孙霄[1] 

机构地区：[1]2512济南山东大学齐鲁医院超声科

出　　处：《中华超声影像学杂志》2017年第7期628-631,共4页Chinese Journal of Ultrasonography

摘　　要：目的探讨不同组织来源的肿瘤细胞系在超声联合微泡作用过程中的实验参数设置差异及对其生物效应的影响。方法选取微泡浓度（A）、超声声强（B）、超声辐照时间（C）三个因素，分别选取三水平（1、2、3），设计L3（3^3）因素三水平的正交试验。根据正交试验表，超声联合微泡分别辐照人乳腺癌MCF-7细胞、人卵巢癌A2780细胞、人肝癌Bel7402细胞、人甲状腺癌ARO细胞，MTT法检测细胞存活率，流式细胞仪检测细胞膜通透性。结果四组细胞的最佳实验参数分别为：乳腺癌MCF-7细胞：A381C1；卵巢癌A2780细胞：A183C3；肝癌Bel7402细胞和甲状腺癌ARO）细胞：A283C2。最佳实验参数下4组细胞的细胞存活率均在75％以上，乳腺癌MCF-7细胞与甲状腺癌ARO细胞间细胞的荧光染色阳性率差异无统计学意义（P=0．487），余各组间差异均有统计学意义（均P〈0．05）。结论不同肿瘤细胞系之间达到最佳转染条件的参数设置存在差异，且最佳参数下产生的生物学效应不同。Objective To explore the bioeffect of different parameters on 4 cell lines by ultrasound mediated microbubble destruction. Methods The orthogonal experimental design was used to investigate the effect of three factors on the bioeffects of four cell lines under three levels. Three factors included microbubble concentration, sound intensity, irradiation time. Human breast tumor （MCF-7） cells, ovarian tumor （A2780） cells, liver tumor （Bel7402） cells and thyroid tumor （ARO） cells were exposed to ultrasound in the presence of SonoVue. The cell survival rate was determined by MTT methods and the cell luminosity factor was detected by flow cytometry. Results The optimum parameters for Bel7402 and ARO cell were the same （A2B3C2）, and they were different from those from MCF 7 （A3B1C1） and A2780 （A1B3C3） cell. The cell survival rates for 4 cell lines were above 75 %, and the cell luminosity factors were different among 4 cell lines. Conclusions The optimum parameters by ultrasound-mediated microbubble destruction for different cell lines are different, and under the optimum parameters the bioeffects of different cell lines are different.

关 键 词：超声疗法 微气泡 生物效应 细胞膜通透性 

分 类 号：R730.5[医药卫生—肿瘤]