电磁环境胁迫对铜绿微囊藻氧化应激和光合固碳酶的影响作用研究  

The effects of the electromagnetic environment on oxidative stress and photosynthetic carbon fixation of Microcystis aeruginosa

作　　者：唐超[1,2] 喻慧[1] 杨传俊[1] 蔡澎[1] 

机构地区：[1]中国科学院城市环境研究所中国科学院城市环境与健康重点实验室,厦门361021 [2]中国科学院大学,北京100049

出　　处：《环境科学学报》2017年第8期3194-3200,共7页Acta Scientiae Circumstantiae

基　　金：中国科学院国防科技创新基金(No.CXJJ-16M115);厦门科技计划项目(No.3502Z20162021)~~

摘　　要：研究了3种典型电磁环境(射频电磁辐射(RF-EMF)、极低频电磁场(ELF-EMF)、交变电场(AEF))胁迫对铜绿微囊藻(Microcystis aeruginosa)细胞氧化应激(超氧化物歧化酶(SOD)、丙二醛(MDA))和光合固碳酶(核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)、果糖-1,6-二磷酸醛缩酶(FBA))的影响作用.结果表明,短期和长期RF-EMF、ELF-EMF及AEF暴露胁迫铜绿微囊藻细胞,总体能极显著提高藻细胞的SOD活性(p<0.01).铜绿微囊藻经RF-EMF、ELF-EMF及AEF短期暴露处理后,均极显著提高了藻细胞内MDA含量(p<0.01);铜绿微囊藻经RFEMF、AEF长期暴露处理后,藻细胞内MDA含量极显著降低(p<0.01).RF-EMF暴露处理铜绿微囊藻,总体能显著提高藻细胞Rubisco酶活性(p<0.05).长期RF-EMF、AEF和ELF-EMF暴露下的铜绿微囊藻,总体表现出FBA酶活性极显著降低(p<0.01).以上结果说明,RF-EMF、ELFEMF、AEF均能够诱导铜绿微囊藻发生氧化应激反应.而RF-EMF能够对铜绿微囊藻细胞参与光合作用的关键酶(Rubisco和FBA)产生一定的调控作用,表明射频电磁波能够在一定程度上影响铜绿微囊藻细胞的光合作用.The effect of the activities of antioxidant enzyme systems（SOD,MDA） and photosynthesis catalytic enzymes（Rubisco,FBA） of Microcystis aeruginosa were studied under the exposure of three kinds of typical electromagnetic environment（radiofrequency electromagnetic fields（RF-EMF）,extremely low frequency electromagnetic fields（ELF-EMF）,alternating electric field（AEF））.The results showed that the activities of SOD in M.aeruginosa cells increased significantly under the RF-EMF,ELF-EMF and AEF under both short and long term（p〈0.01）.The contents of MDA in M.aeruginosa cells increased significantly under the short term exposure of RF-EMF,ELF-EMF,and AEF（p〈0.01）.The contents of MDA in M.aeruginosa cells decreased significantly under the sustainable exposure of RF-EMF and AEF（p 0.01）.The activity of Rubisco in M.aeruginosa cells increased significantly under the exposure of RF-EMF（p〈0.05）.The long term exposure of RF-EMF,AEF and ELF-EMF to M.aeruginosa significantly reduced FBA activity（p〈0.01）.The results mentioned above indicated that oxidative stress of M.aeruginosa could be induced under the exposure of RF-EMF,ELF-EMF and AEF.And the regulations on key enzymes of photosynthesis（Rubisco and FBA） by RF-EMF implied that radiofrequency electromagnetic fields could affect the photosynthesis of M.aeruginosa cells to some extent.

关 键 词：电磁环境 铜绿微囊藻 氧化应激 光合作用 

分 类 号：X171.5[环境科学与工程—环境科学]