肝癌相关抗原动力素蛋白D2和D3选择剪接区在广西人肝癌组织表达的差异性研究  

Differential expression of D2 and D3 alternative splicing sites of hepatocellular carcinoma-associated antigen kinectin in human hepatocellular carcinoma tissues and normal liver tissues in Guangxi Province

作　　者：闫磊[1] 吴红[1] 李凯军[1] 张绪东[1] 李月珍[1] 李爽[1] 胡静[1] 金花淑[1] 罗国容[2] Yan L Wu H Li K J Zhang XD Li YZ Li S Hu J Jin HS Luo GR(Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, China Department of Histology and Embryology, Guangxi Medic~d University, Nanning 530021, China)

机构地区：[1]黑龙江省牡丹江医学院组胚学教研室,牡丹江157011 [2]广西医科大学组胚学教研室,南宁530021

出　　处：《中华地方病学杂志》2017年第8期562-565,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81160264、81060168）;黑龙江省教育厅科学技术研究项目（11551529）

摘　　要：目的观察动力素蛋白（kineetin）D2、D3选择剪接区（简称D2、D3区）在广西人肝癌组织、癌旁组织以及正常肝组织中表达的差异性，探索kinectin的选择剪接区与肝癌发生之间可能存在的联系。方法在广西医科大学第一附属医院肝胆外科收集45例广西籍肝癌患者癌组织与相应癌旁组织标本，以及10例正常肝组织标本，采用半定量PCR法检测D2、D3区表达的差异性，并进行统计学分析。结果D2L/D2S（包含D2区的长片段／不包含D2区的短片段）表达比值肝癌组织为2．709±1．025，癌旁组织为1．564±0．357，正常肝组织为1．507±0．499，组间比较差异有统计学意义（F=29．698，P〈0．05），其中肝癌组织D2L／D2S表达比值明显高于癌旁组织和正常肝组织（P均〈0．05）；D3L／D3S（包含D3区的长片段／不包含D3区的短片段）表达比值肝癌组织为1．232±0．041，癌旁组织为1．156±0．309，正常肝组织为1．282±0．343，组问比较差异无统计学意义（F=0．989，P〉0．05）。结论包含D2区的kinectin异型体在肝癌组织中有过度表达，提示其可能与肝癌的发生有一定的相关性．Objective To analyze the differential expression of D2 and D3 alternative splicing sites of human kinectin in human hepatocellular carcinoma （HCC） tissues, adjacent non-cancerous tissues and normal liver tissues, and to investigate a possible relationship between alternative splicing sites of kinectin and hepatocarcinogenesis. Methods The cDNA was obtained by RT-PCR in 45 coupled HCC cancerous and adjacent tissues, and 10 normal liver tissues. The difference in the expression of D2 and D3 alternative splicing sites in cDNA was examined by semi-quantitative PCR, and statistical analysis was performed. Results The ratio of D2L （long segment contains of the D2 region）/D2s （short segment that does not contain a D2 zone） in hepatocellular cancerous tissues was 2.709 ± 1.025, the ratio of D2L/D2S in adjacent non-cancerous tissues was 1.564± 0.357, and the ratio of D2L/D2S in normal liver tissues was 1.507 ± 0.499. The differences were statistically significant （F = 29.698, P 〈 0.05）; the ratio of D2L/ D2s in hepatocellular cancerous tissues was higher than that of adjacent non-cancerous tissues and normal liver tissues （P 〈 0.05）. The ratio of D3L （long segment contains of the D3 region）/D3s （short segment that does not contain a D3 zone） in hepatocellular cancerous tissues was 1.232 ± 0.041, the ratio of D3L/D3s in adjacent non-cancerous tissues was 1.156 ± 0.309, and the ratio of D3L/D3S in normal liver tissues was 1.282 ± 0.343. The ratio of D3L/D3S was not significantly different among hepatocellular cancerous tissues, adjacent non-cancerous tissues and normal liver tissues （F = 0.989, P 〉 0.05）. Conclusion Variant containing D2 is over expressed in cancerous tissues and this alteration may be tumor associated.

关 键 词：动力素蛋白 选掸剪接区 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]