转DBN9978基因抗虫抗除草剂玉米对大鼠的亚慢性毒性研究  

Subchronic Toxicity Test of Genetic Modified Corn with DBN9978 Gene in Rats

作　　者：王睿[1] 胡贻椿[1] 李敏[1] 吴景欢[1] 杨晓光[1] 毛德倩[1] WANG Rui HU Yi-chun LI Min WU Jing-huan YANG Xiao-guang MAO De-qian(National Institute for Nutrition andHealth, Chinese Center for Disease Control and Prevention/ Key Laboratory of Trace Element Nutrition, National Health and Family Planning Commission, Beijing 100050, China)

机构地区：[1]中国疾病预防控制中心营养与健康所/国家卫计委微量元素营养重点实验室,北京100050

出　　处：《中国食物与营养》2017年第6期12-17,共6页Food and Nutrition in China

基　　金：国家科技重大专项--转基因生物新品种培育"抗虫抗除草剂转基因玉米食品安全评价研究"

摘　　要：目的:研究喂饲转DBN9978基因玉米对大鼠的亚慢性毒性。方法:选用断乳SD大鼠140只,随机分为7组,即3个转基因组(73.3%、36.65%、18.33%)、3个亲本玉米组(72.7%、35.35%、18.18%)、1个常规基础饲料对照组。每组20只,雌雄各半。动物2只/笼喂养,自由进食饮水,连续观察90d。观察大鼠体重、进食量、血液学指标、血生化指标,实验结束后摘取动物脏器进行病理组织学检查。结果:各剂量组动物的体重均呈增长趋势,转基因组、亲本对照组与饲养对照组每周体重及体重总增重、每周进食量、总食物利用率以及中、末期的血液学和血生化指标,各主要脏器系数及病理组织学检查比较,均未发现有生物学意义的改变。结论:现有试验结果不能证实该转DBN9978基因玉米对大鼠有亚慢性毒性作用。[Objective] The subchronic toxicity of feed with DBN9978 genetic modified corn was tested in rats. [Method] A total of 140 SD rats were randomly divided into 7 groups with 20 rats each group including three transgenic corn groups with different doses （73. 3% , 36. 65% , 18. 33% ）, three containing non-transgenic corn （72. 7% , 35. 35% , 18. 18% ）, 1 routine Basal diet control group. Animals 2/cage feeding, free to eat drinking water, continuous observation of 90 days. Observation of body weight, food intake, the blood routine test, blood biochemistry test （at the 45 th day and the 90 th day of the experiment）, organ weight and organ pathological examination （at the end of the 90 th day） were conducted. [Result] The weight of the animals in each dose group showed an increasing trend. The weight and weight gain, weekly food intake, total food utilization rate and blood and blood biochemical indexes of the transgenic corn groups, con- taining non-transgenic corn group and the feeding control group, The main organ coefficient and histopathological examination were found no significant changes in biological significance. [Conclusion] There were no enough evidences to confirm GM corn with DBN9978 genes has subchronic toxic effects on rat.

关 键 词：转基因玉米 DBN9978基因 亚慢性毒性 

分 类 号：TS201.6[轻工技术与工程—食品科学]