苯乙醇苷类化合物与ctDNA的相互作用  

Interaction of phenylethanoid glycosides and calf thymus DNA

作　　者：袁欢[1] 王涛[1] 祝晨蔯[2] 吴爱芝[1] 林朝展[2] YUAN Huan WANG Tao ZHU Chen-chen WU Ai-zhi LIN Chao-zhan(School of Chinese Materia Medica , Guangzhou University of Chinese Medicine, Guangzhou 510006, China Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学临床药理研究所,广东广州510405

出　　处：《中国中药杂志》2017年第14期2725-2729,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81603269;81573566);广东省自然科学基金项目(2015A030310491)

摘　　要：以中性红为分子探针研究2种苯乙醇苷类化合物(PhGs)毛蕊花糖苷和连翘酯苷B在生理条件下与小牛胸腺DNA(ctDNA)之间的相互作用。荧光光谱、紫外光谱、黏度、DNA热变性实验和分子对接结果表明:这2种PhGs主要采用氢键作用以沟区方式结合到ctDNA双螺旋小沟中,毛蕊花糖苷与DNA结合作用更强。In physiological condition, the interaction of acteoside and forsythoside B with calf thymus DNA using neutral red （NR） as a fluorescence probe were investigated by fluorescence, UV-visible spectrophotometry, viscosity, DNA melting techniques, and mo- lecular docking. It is observed that acteoside and forsythoside B can react with DNA. The major mode of recognition between drug and DNA is groove binding by hydrogen bonds, and the interaction of acteoside with DNA is stronger than that of forsythoside B.

关 键 词：苯乙醇苷 CTDNA 中性红 分子对接 

分 类 号：R285[医药卫生—中药学]