基于PPARs信号通路探索CB1对脂质代谢的调节作用  

Cannabinoid receptor 1 in regulation of lipid metabolism through PPARs pathway

作　　者：魏立雯 王丕森 赵明德 古从伟 韩建红 袁章琴[2] 付陆 

机构地区：[1]西南医科大学,泸州646000 [2]苏州大学附属第一医院骨科研究所,215006

出　　处：《国际医药卫生导报》2017年第15期2326-2329,共4页International Medicine and Health Guidance News

基　　金：四川省教育厅基金资助项目（14ZA0146）;四川省泸州市-泸州医学院联合基金资助项目（2013LZLY-J46）

摘　　要：目的研究CB1对PPARs信号通路在高脂饮食诱导肥胖小鼠脂质代谢的调节作用。方法构建小鼠肥胖模型，观察给予CB1抑制剂利莫那班后小鼠体重、肝脏重量及血清生化指标的变化，并检测CB1、PPARd、PPARB和PPARγ基因在各组织mRNA水平的表达情况。结果利莫那班降低了小鼠的体重和肝脏重量（P〈0．05）；改善了血清生化指标（P〈0．05）；各组织中CB1基因mRNA水平的表达降低（P〈0．05）；PPARα、PPARγ基因在皮下脂肪、内脏脂肪、肝脏组织mRNA水平的表达显著增高（P〈0．05）；PPARβ基因在各组织mRNA水平表达情况差异无统计学意义。结论CB1通过作用于PPARα、PPARγ调节脂质代谢，为临床上脂质代谢紊乱的治疗提供了另一个可靠的理论依据。Objective To study the role of cannabinoid receptor 1 （CB1） in the regulation of lipid metabolism through PPARs pathway in diet-induced obese mice. Methods The C57BL/6J male mice were induced with high-fat diet, and then we detected the effect of rimonabant on the body weight, liver weight, and biochemical indicators of the mice. The mRNA levels of CB1, PPARα, PPARβ, and PPARγ in subcutaneous fat, visceral fat, skeletal muscle, liver, and heart were detected. Results Rimonabant could decrease the body weight, liver weight, and the mRNA levels of CB1 significantly （all P 〈 0.05）. It also ameliorated the serum levels of biochemical indicators （P 〈 0.05）. It increased the mRNA levels of PPARα and PPARy in subcutaneous fat, visceral fat, and liver. However, no statistical differences in PPARβ were found in each tissue. Conclusions CB1 plays a role in the regulation of lipid metabolism by controlling PPARα and PPARγ. It provides a reliable theoretical basis for the clinical treatment of lipid metabolism disorders.

关 键 词：CB1 利莫那班 PPARS 肥胖 脂质代谢 

分 类 号：R589.2[医药卫生—内分泌]