卡铂短期诱导人卵巢癌类干细胞的分离及初步鉴定  

Isolation and Identification of Human Ovarian Cancer Stem-like Cells Induced by Short-term Treatment with Carboplatin

作　　者：王宁[1] 倪莎[1] 周欣[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医科大学学报》2017年第8期713-719,共7页Journal of China Medical University

基　　金：辽宁省教育厅重点实验室项目(LS201602);沈阳市科技创新专项资金-人口与健康科技攻关专项(F15-139-9-34)

摘　　要：目的分离卡铂耐药型人卵巢癌类干细胞,为研发和筛选针对卡铂耐药的卵巢癌二线治疗药物提供细胞模型。方法从卵巢癌组织或腹水中分离培养卵巢癌细胞,直接或采用卡铂短期诱导后无血清悬浮培养,分离纯化获得卵巢癌类干细胞球,采用免疫荧光法或流式细胞术检测干细胞标志物的表达。结果卵巢癌原代细胞直接或采用卡铂短期诱导后无血清悬浮培养能够形成类干细胞球,并高表达干细胞标记物CD133,ALDH1,ABCG2和CD24。卡铂短期诱导分离的卵巢癌干细胞球中CD24+细胞含量明显增加。结论原代培养卵巢癌细胞经卡铂短期诱导后无血清悬浮培养,能进一步富集纯化卵巢癌类干细胞,可作为卵巢癌耐药研究的理想细胞模型。Objective To isolate carboplatin-resistant human ovarian cancer stem-like cells and provide a cellular model for the development and screening of second-line drugs for carboplatin-resistant ovarian cancer. Methods Primary ovarian cancer cells were isolated and cultured from ovarian cancer tissues and ascites, and ovarian cancer stem-like cell spheres were isolated from serum-free suspension culture directly or after car- boplatin induction. The expression of stem cell markers was evaluated by immunofluorescent assay or flow cytometry. Results The ovarian cancer stem-like cell spheres were isolated successfially from both ovarian cancer tissues and ascites and expressed high levels of stem cell markers CD133, ALDH1, ABCG2, and CD24. The abundance of CD24＋ cells in the ovarian cancer stem cells was significantly increased upon short-term induction with carboplatin. Conclusion Ovarian cancer stem-like cells can be enriched and purified by short-term induction with carboplatin. This cell model can be used in research on chemoresistant ovarian cancer in the future.

关 键 词：卵巢癌 无血清悬浮培养 卵巢癌类干细胞球 卡铂 

分 类 号：R364.7[医药卫生—病理学]